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德国Nova刚地弓形虫第二代检测试剂盒

德国Nova刚地弓形虫第二代检测试剂盒

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Toxoplasma gondii is a ubiquitous protozoon that has a worldwide distribution. This obligate intracellular parasite can infect humans as well as virtually all warm-blooded animals, including mammals a

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德国Nova刚地弓形虫第二代检测试剂盒

刚地弓形虫是一种无处不在的原虫,世界范围内的分布。这个专性细胞内寄生虫可以感染人类,以及几乎所有的温血动物,包括哺乳动物和鸟类。弓形虫的生命周期是兼性heteroxenous。中间宿主可能是所有温血动物包括大部分牲畜,和人类。明确的主机是猫科家族的成员,例如家猫。

有三个感染弓形虫的生命周期阶段,即个速、bradyzoites(在组织囊肿),和子孢子(形成孢子卵囊)。所有三个阶段都是传染性中间和zui终的主机。

弓形虫感染可能是收购主要是通过以下路线之一:口服摄入的传染性卵囊的环境,通过口服摄入的组织中包含的囊肿中间宿主的生的或未煮熟的肉类,或经胎盘的个的传播。

虽然在人类感染弓形虫很常见,临床疾病在很大程度上局限于高危人群。大多数感染弓形虫免疫活性的人类是无症状的。

然而,如果*次怀孕期间感染,弓形虫可能会传染给胎儿。先天性弓形体病可能导致流产、新生儿死亡或胎儿异常对胎儿有害的后果。如果不及时治疗,胎儿的宫内感染风险增加在怀孕期间。

疾病

症状

感染的机制

后天获得的弓形体病

免疫活性的患者:

  • 通常无症状或非常温和
  • 非特异性的症状包括颈部淋巴结病和其他感染广义的症状(发热、不适、盗汗,肌痛,喉咙痛和斑丘疹的皮疹)

摄入肉或内脏组织囊肿感染

重新激活弓形体病

免疫功能低下的患者:

  • 脑炎、心肌炎、肺炎

卵囊在猫科动物粪便污染的食物或水

先天性弓形体病

先天感染新生儿:

  • 脉络膜视网膜炎、失明、癫痫、精神发育迟滞、贫血、黄疸、脑炎、肺炎等
  • *恢复是非常罕见的

先天性传播:如果怀孕期间发生感染的急性期,这种寄生虫可以穿过胎盘和胎儿感染


感染的诊断则需要通过:

  • 血清学:确定特定抗体的ELISA、间接荧光抗体试验(IFAT)免疫吸附凝集试验(ISAGA)
  • 寄生虫检测:聚合酶链反应

NovaLisa®刚地弓形虫免疫球蛋白g ELISA:

NovaLisa®刚地弓形虫免疫球蛋白g ELISA定量测定的目的是IgG-class刚地弓形虫抗体在人类血清或血浆(柠檬酸)


NovaLisa®刚地弓形虫IgMµ-capture ELISA:

NovaLisa®刚地弓形虫IgMµ-capture ELISA用于定性测定IgM-class刚地弓形虫抗体在人类血清或血浆(柠檬酸)

抗原:

刚地弓形虫溶解产物(免疫球蛋白),刚地弓形虫嵌合抗原(IgM)

具体的性能特征:

 

Intraassay

Interasay

灵敏度%

特异性%

 

n

的意思是

CV %

n

的意思是

CV %

 

 

免疫球蛋白

6

0.978

5.7

4

0.752

7.1

96.6

98.2

6

1.647

5.3

4

1.26

10.1

6

2.496

6.2

4

1.98

6.3

IgM
µ-capture

24

1.563

2.9

12

7.08

6.5

95.8

> 98

24

0.304

1.5

12

16.68

4.5


NovaLisa®刚地弓形虫免疫球蛋白g贪欲测试*:

贪欲的决心是一种诊断方法,用于区分zui近(急性)和更遥远的(过去的)病人血清感染刚地弓形虫。贪欲的约束力是抗体与相应抗原(血清标本)

免疫球蛋白的测定贪欲依赖的进步增加亲和力的抗体靶抗原的过程中天然免疫后感染。测量抗体绑定的强度是一种证实或排除近期感染。Low-avidity抗体从zui近收购了弓形虫感染刚地可以从high-avidity分化抗体,这是一个指示性的感染。

换句话说,低的免疫球蛋白抗体在感染的早期阶段可以从高分化的抗体与过去的感染有关。

IgG抗体活性的测定是一个额外的分析经典的血清学关于刚地弓形虫感染的状态。

NovaLisa®刚地弓形虫免疫球蛋白g贪欲测试是一种额外的试剂盒与NovaLisa结合使用®刚地弓形虫免疫球蛋白g ELISA


性能特点:

NovaLisa®刚地弓形虫免疫球蛋白g贪欲测试被用于评估弓形体病急性和过去感染样品。总数量86定义病人样本测试。这些样本提供的医学微生物学研究所免疫学和寄生虫学,波恩大学。



 

定义样本

 

急性感染

过去的感染

协议
(%)

NovaLisa®刚地弓形虫免疫球蛋白g贪欲测试

急性感染

36

1

37

94.7

过去的感染

2

47

49

97.9

38

48

86

96.5


订单信息:

ELISA

的数量决定

产品编号

弓形虫免疫球蛋白

96

TOXG0460

弓形虫IgMµ-capture

96

TOXM0460

弓形虫免疫球蛋白g贪欲测试*

96

TOXGA460

* NovaLisa®刚地弓形虫免疫球蛋白g贪欲测试是一种额外的试剂,用于结合NovaLisa®弓形虫刚免疫球蛋白ELISA


RiliBAK

Liquickeck®火炬+控制(Bio-Rad)外部控制可用NovaLisa®刚地弓形虫免疫球蛋白g

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Toxoplasma gondii 2nd Generation

Toxoplasma gondii is a ubiquitous protozoon that has a worldwide distribution. This obligate intracellular parasite can infect humans as well as virtually all warm-blooded animals, including mammals and birds. The life cycle of T. gondii is facultatively heteroxenous. Intermediate hosts are probably all warm-blooded animals including most livestock, and humans. Definitive hosts are members of the family Felidae, for example domestic cats.

There are three infectious stages in the life cycle of T. gondii, i.e. tachyzoites, bradyzoites (in tissue cysts), and sporozoites (in sporulated oocysts). All three stages are infectious for both intermediate and definitive hosts.

T. gondii infections may be acquired mainly via one of the following routes: by oral ingestion of infectious oocysts from the environment, by oral ingestion of tissue cysts contained in raw or undercooked meat of intermediate hosts, or by transplacental transmission of tachyzoites.

While infection with T. gondii in humans is very common, clinical disease is largely confined to risk groups. Most T. gondii infections in immunocompetent humans are asymptomatic.

However, if first contracted during pregnancy, T. gondii may be transmitted to the foetus. Congenital toxoplasmosis may cause abortion, neonatal death, or foetal abnormalities with detrimental consequences for the foetus. If not treated, the risk of intrauterine infection of the foetus increases during pregnancy.

Disease

Symptoms

Mechanism of Infection

Postnatally acquired toxoplasmosis

Immunocompetent patients:

  • Usually asymptomatic or very mild
  • Nonspecific symptoms including cervical lymphadenopathy and other symptoms of generalized infection (fever, malaise, night sweats, myalgia, sore throat and maculopapular rash)

Ingestion of tissue cysts in infected meat or viscera

 

Reactivated toxoplasmosis

Immunocompromised patients:

  • encephalitis, myocarditis and pneumonitis

Oocysts in food or water contaminated with feline faeces

Congenital toxoplasmosis

Congenitally infected newborns:

  • chorioretinitis, blindness, epilepsy, mental retardation, anemia, jaundice, encephalitis, pneumonitis and others
  • Complete recovery is very rare

Congenital transmission: if the acute phase of the infection occurs during pregnancy, the parasite can cross the placenta and infect the fetus


Infections may be diagnosed by:

  • Serology:    Determination of specific antibodies by ELISA, indirect fluorescence antibody test (IFAT) immunosorbent agglutination assay (ISAGA)
  • Parasite detection: PCR

NovaLisa® Toxoplasma gondii IgG ELISA:

The NovaLisa® Toxoplasma gondii IgG ELISA is intended for the quantitative determination of IgG-class antibodies against Toxoplasma gondii in human serum or plasma (citrate).


NovaLisa® Toxoplasma gondii IgM µ-capture ELISA:

The NovaLisa® Toxoplasma gondii IgM µ-capture ELISA is intended for the qualitative determination of IgM-class antibodies against Toxoplasma gondii in human serum or plasma (citrate).

Antigens:

Toxoplasma gondii lysate (IgG) and Toxoplasma gondii chimeric antigens (IgM)

Specific performance characteristics:

 

Intraassay

Interasay

Sensitivity%

Specificity%

 

n

Mean

CV%

n

Mean

CV%

 

 

IgG

6

0.978

5.7

4

0.752

7.1

96.6

98.2

6

1.647

5.3

4

1.26

10.1

6

2.496

6.2

4

1.98

6.3

IgM 
µ-capture

24

1.563

2.9

12

7.08

6.5

95.8

>98

24

0.304

1.5

12

16.68

4.5


NovaLisa® Toxoplasma gondii IgG Avidity Test*:  

The avidity determination is a diagnostic method which is used to differentiate a recent (acute) and a more distant (past) infection with Toxoplasma gondii in patient sera. Avidity is the binding force of the antibody (serum specimen) with the corresponding antigen. 

The determination of IgG avidity relies on the progressive increase of the affinity of an antibody for its target antigen during the course of natural immunity following an infections. Measuring the strength of the antibody binding is a way to confirm or rule out a recent infection. Low-avidity antibodies from a recent acquired Toxoplama gondii infection can be differentiated from high-avidity antibodies, which is an indicative of past infection.

In other words, low avid IgG antibodies in the early stage of infection can be differentiated from high avid antibodies associated with a past infection.

The determination of IgG antibody avidity is an additional analysis to the classic serology in regards to the status of a Toxoplasma gondii infection.

The NovaLisa® Toxoplasma gondii IgG Avidity Test is an additional reagent-kit that has to be used in combination with NovaLisa® Toxoplasma gondii IgG ELISA.  


Performance Characteristics:

The NovaLisa® Toxoplasma gondii IgG Avidity Test has been evaluated for use in Toxoplasmosis with samples of acute and past infections. A total number of 86 defined patient samples were tested. These samples were supplied by the Institute of Medical Microbiology, Immunology and  Parasitology, University Bonn.  



 

Defined Samples

 

Acute Infection

Past Infection

Total

Agreement
(%)

NovaLisa® Toxoplasma gondii IgG Avidity Test

Acute Infection

36

1

37

94.7

Past Infection

2

47

49

97.9

Total

38

48

86

96.5


Order information:

ELISA

Number of Determinations

Product Number

Toxoplasma IgG

96

TOXG0460

Toxoplasma IgM µ-capture

96

TOXM0460

Toxoplasma IgG Avidity Test*

96

TOXGA460

* The NovaLisa® Toxoplasma gondii IgG Avidity Test is an additional reagent that has to be used in combination with the NovaLisa® Toxoplama gondii IgG ELISA.


RiliBÄK

With Liquickeck® ToRCH Plus Control (Bio-Rad) an external control is available for the NovaLisa® Toxoplasma gondii IgG

 

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