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贪欲刚地弓形虫检测试剂盒

贪欲刚地弓形虫检测试剂盒

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刚地弓形虫是一种小型细胞内寄生虫的生活循环性和一个无性阶段。性发育仅限于(可能只)猫的肠细胞;卵囊形成分泌和抗由于其细胞壁他们可能感染在有利的情况下至少1年。动物和人是弓形虫的中间宿主的无性增殖:摄取寄生虫宿主细胞内增殖爆炸将Z终溶解它们。他们传播通过循环和全身淋巴系统虽然可以感染任何细胞类型。在肌肉和大脑细胞囊肿形成球状,直径约5 - 100μm。

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贪欲刚地弓形虫的第二代

刚地弓形虫是一种小型细胞内寄生虫的生活循环性和一个无性阶段。性发育仅限于(可能只)猫的肠细胞;卵囊形成分泌和抗由于其细胞壁他们可能感染在有利的情况下至少1年。动物和人是弓形虫的中间宿主的无性增殖:摄取寄生虫宿主细胞内增殖爆炸将zui终溶解它们。他们传播通过循环和全身淋巴系统虽然可以感染任何细胞类型。在肌肉和大脑细胞囊肿形成球状,直径约5 - 100μm。囊肿在中间居屋计划几乎是不朽的

刚地弓形虫是zui常见的寄生虫在人体内,但其丰度(7 - 80)是高度依赖于地理区域,社会经济地位和营养海关。感染很少引起弓形虫病,通常没有临床症状,但可能在免疫抑制者和胎儿产生严重的问题。

因为只有一个主怀孕期间感染可能是危险的,甚至是致命的未出生的(先天性感染的概率大约是50%),zui近爆发的感染必须被排除在外。

在超过98%的情况下,孕妇缺乏IgM排除近期感染的可能性。在新生儿anti-toxoplasma IgM的存在就足以证实先天性弓形体病,孕产妇IgM以来,与免疫球蛋白,不穿过胎盘屏障。但大量感染婴儿不开发检测IgM水平,因此是假阴性。在免疫抑制患者弓形体病导致严重并发症主要由活化的早期潜伏性感染。

物种

疾病

症状

感染的机制

弓形虫Godndii

??体病

获得Toxopasmosis:
淋巴结病,脉络膜视网膜炎

先天性弓形体病:
头小畸型脑积水,颅内钙化,慢性的脉络膜视网膜炎

由食物摄入的卵囊,包括猫的粪便或受污染的土壤的污染水

摄入的囊肿生吃或煮熟的肉不够,特别是猪肉

先天性感染


感染的诊断则需要通过:

  • PCR(病毒RNA的检测)
  • 环境影响评价,ELISA(特定抗体的检测)。

免疫球蛋白g刚地弓形虫抗体的存在表明感染的发生,但不区分近期和过去的感染。特异性IgM抗体是*检测到十天和峰值大约4周后感染。他们可能持续超过七个月后急性感染?;谥ぞ荼砻骺固寤疃灾鸾ピ黾颖┞逗竺庖咴?/span>,贪欲的免疫球蛋白抗体可以用作标记区分近期主要从长期感染。贪欲描述特定的抗体对抗原的结合强度。Low-avidity免疫球蛋白抗体表明原发感染,而免疫球蛋白抗体的存在与高活动性指向持久性或继发感染。

打算使用

NovaLisa®贪欲刚地弓形虫免疫球蛋白g ELISA旨在表明t gondii-specific免疫球蛋白热望在人类血清或血浆(肝素,柠檬酸)区分急性和过去的感染


性能特点:

NovaLisa®贪欲刚地弓形虫免疫球蛋白g测试已经评估了使用与样品弓形体病急性和过去的感染。总数量84定义病人样本测试。这些样本提供的医学微生物学研究所免疫学和寄生虫学,波恩大学。



 

定义样本

 

低的热望

高活动性

协议
(%)

NovaLisa®贪欲刚地弓形虫免疫球蛋白g测试

低的热望

46

0

46

100

高活动性

0

38

38

100

46

38

84

100


订单信息:

ELISA

的数量决定

产品编号

弓形虫免疫球蛋白

96

TOXG0460

弓形虫IgMµ-capture

96

TOXM0460

弓形虫免疫球蛋白g贪欲测试

96

ATOX7460

 

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Avidity Toxoplasma gondii 2nd Generation

Toxoplasma gondii is a small intracellular parasite, whose live cycle has a sexual and an asexual phase. Sexual development is restricted to the intestinal cells of (probably exclusively) cats; the oocysts formed are excreted and due to their resistant cell walls they may be infectious under advantageous circumstances for at least 1 year. Animals and man are intermediate hosts for the asexual proliferation of T. gondii: the ingested parasites will proliferate explosively within the host cells lysing them eventually. They disseminate throughout the body via circulation and lymphatic system and though may infect any cell type. In muscle and brain cells cysts are formed which are spheroidal and about 5-100 μm in diameter. Cysts are virtually immortal in the intermediate hos

Toxoplasma gondii is the most common parasite in humans, but its abundance (7-80 %) is highly dependent on the geographic area, the socioeconomic status and the nutritional customs. Infection only rarely causes toxoplasmosis and usually clinical symptoms are absent, but may produce severe problems in immunosuppressed persons and fetus.

Because only a primary infection during pregnancy may be dangerous and even fatal for the unborn (the probability of congenital infection is about 50 %), the recent onset of an infection must be excluded.

In pregnant women in over 98 % of cases, the absence of IgM excludes the possibility of recent infection. In newborns the very presence of anti-toxoplasma IgM is sufficient to confirm a congenital toxoplasmosis, since maternal IgM, unlike IgG, does not cross the placental barrier. But a significant number of infected infants do not develop detectable IgM levels and thus are false negative. In immunosuppressed patients toxoplasmosis causes severe complications mostly by reactivation of an earlier latent infection.

 

Species

Disease

Symptoms

Mechanism of Infection

Toxoplasma Godndii

Toxoplasmosis

Acquired Toxopasmosis:
lymphadenopathy, chorioretinitis

Congenital Toxoplasmosis:
hydrocephalus, microcephaly, intracranial calcifications, chronical chorioretinitis

Ingestion of oocysts by food, including water contaminated feces of cats or contaminated soil

Ingestion of cysts by eating raw or insufficiently cooked meat, esp. pork

Congenital infection


Infections may be diagnosed by:  

  • PCR (detection of viral RNA)
  • EIA, ELISA (detection of specific antibodies).

The presence of IgG antibodies to Toxoplasma gondii indicates the occurrence of the infection but does not distinguish between recent and past infection. Virus-specific IgM antibodies are first detected ten days and peak at about four weeks post infection. They may persist for more than seven months after acute infections. Based on the evidence that antibody avidity gradually increases after exposure to an immunogen, avidity of IgG antibodies can be used as a marker for distinguishing recent primary from long-term infections. Avidity describes the binding strength of a specific antibody to its antigen. Low-avidity IgG antibodies indicate a primary infection, whereas the presence of IgG antibodies with high avidity points to persistency or reactivation of infection.

Intend Use

The NovaLisa® Avidity Toxoplasma gondii IgG ELISA is intended to indicate the T. gondii-specific IgG avidity in human serum or plasma (citrate, heparin) to differentiate between acute and past infection


Performance Characteristics:

The NovaLisa® Avidity Toxoplasma gondii IgG Test has been evaluated for use in Toxoplasmosis with samples of acute and past infections. A total number of 84 defined patient samples were tested. These samples were supplied by the Institute of Medical Microbiology, Immunology and  Parasitology, University Bonn.  



 

Defined Samples

 

Low Avidity

High Avidity

Total

Agreement
(%)

NovaLisa® Avidity Toxoplasma gondii IgG Test

Low Avidity

46

0

46

100

High Avidity

0

38

38

100

Total

46

38

84

100


Order information:

ELISA

Number of Determinations

Product Number

Toxoplasma IgG

96

TOXG0460

Toxoplasma IgM µ-capture

96

TOXM0460

Toxoplasma IgG Avidity Test

96

ATOX7460

 

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