疟原虫检测抗原试纸

广州健仑生物科技有限公司

产品应用免疫层析技术（ICT）。一个试剂条中应用了2种不同单克隆抗体，分别来检验血样中是否有 HRPII抗原（恶性疟*）及“泛疟疾抗原” （Pan-malaria）（四种疟原虫共有）。
两种不同的单克隆抗体以及另外一种质控抗体，被固定在膜支撑物上，形成了三条不同的线。膜支撑物与加样垫相连，加样垫浸渍了已经结合可视粒子的质控抗体与抗－疟原虫抗体。结合在一起的膜支撑物与加样垫组成了完整的检测条。该检测条与洗脱垫与吸收垫被一起置于一个书形、铰链状的检测卡中。这就构成了一个完整的快速检测试剂卡。检测时，将全血加到加样垫上，血样中存在的疟原虫抗原与抗疟原虫抗体结合物结合。将A 试剂液加到检测条的底部，使抗原结合物复合物沿检测条流动。固定的抗－疟原虫抗体捕获抗原结合物，形成检测线。固定的质控抗体捕获质控结合物，形成质控线。一旦血样流过检测条，将检测卡闭合，使加到冲洗垫中的A 试剂把检测条上多余的血液洗去。

疟原虫检测抗原试纸

Applications Immunochromatography (ICT). Two different monoclonal antibodies were used in a single reagent strip to test for the presence of the HRPII antigen (unique to P. falciparum) and Pan-malaria (common to all four plasmids) in the blood sample.
Two different monoclonal antibodies, along with another control antibody, were immobilized on the membrane support to form three distinct lines. The membrane support was attached to a sample pad, and the sample pad was impregnated with a control antibody and anti-Plasmodium antibody that had bound to the visual particles. The combined membrane support and coupon make up a complete test strip. The test strip and the elution pad and absorbent pad are placed together in a book-shaped, hinged test card. This constitutes a complete rapid test reagent card. At the time of testing, whole blood is added to the pad, and the Plasmodium antigen present in the blood sample binds to the anti-Plasmodium antibody conjugate. Add reagent A to the bottom of the test strip and allow the antigen-binding complex to flow along the test strip. Immobilized anti-Plasmodium antibodies capture the antigen conjugates to form the assay line. Fixed control antibodies capture the control conjugate to form a control line. Once the blood sample is passed through the test strip, the test card is closed and the A reagent applied to the washpad removes the excess blood from the test strip.

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Symptoms and signs
Ebola virus disease incubation period of 2 days to 21 days. The disease mainly to liver, spleen and kidney and other organs damage. The main symptoms are fever, extreme weakness, muscle aches, headache and sore throat symptoms. Vomiting, diarrhea, rashes, impaired kidney and liver function, followed by internal and external bleeding may occur in some cases. Laboratory tests included a decrease in white blood cell count and a decrease in plaet count, while liver enzymes increased.
The disease in different periods after the onset of different performance:
2 to 3 days of onset of vomiting, nausea, diarrhea, mucus or bloody stool, and may have diarrhea for several days.
4 ~ 5 days duration has entered the extreme period, bleeding in the extreme is a common occurrence, usually vomiting, melena, bleeding at the injection site, nose bleeding, hemoptysis, etc., with special attention is that pregnant women will appear abortion and postpartum Bleeding; In addition, will continue to fever, awareness will change; may be complicated by myocarditis, pneumonia and so on.
The course of 6 to 7 days may occur, with shoulder, palms, palmar-based measles-like rash, systemic organs may have been spread to, and in a few days after scaling.
8 to 9 days course of disease in some critically ill patients or patients with severe complications may die during this period, mainly due to liver and kidney failure.
If the virus persists in semen, it can cause orchitis, testicular atrophy and so on.
Ebola virus disease diagnosis
Other diseases that should be ruled out before a possible diagnosis of Ebola virus disease can be made includes malaria, typhoid, Shigella, cholera, leptospirosis, plague, rickettsial disease, relapsing fever, meningitis, hepatitis and Other viral hemorrhagic fever.