EB病毒早期抗原IgG免疫荧光玻片试剂盒

EBV Early Antigens IgG IFA Kit

广州健仑生物科技有限公司

主要用途：用于检测人血清中的EB病毒早期抗原IgG抗体

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EB病毒早期抗原IgG免疫荧光玻片试剂盒

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【公司名称】 广州健仑生物科技有限公司
【】    杨永汉 
【】 
【腾讯 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-3室
【企业文化】

起初，我们没有获得多大的成功，但在2005年，我们在技术上取得了突破。以前，我们实验室在培养干细胞时，细胞只能平铺在培养皿上，但在2005年，我们突破了“二维限制”，可以让干细胞悬浮在培养液中，这就是“悬浮培养”。我们采用这种三维培养技术的原因有很多。首先，在悬浮培养中，细胞聚集时，本身就会形成三维结构，因此在产生复杂组织时，会比平铺的细胞层更容易;其次，为了发育成复杂的结构，细胞之间需要相互交流，而三维培养更适于促进这样的交流，因为细胞之间可以更加灵活地发生相互作用。
使用这种新方法，我们把相互分离的胚胎干细胞悬浮在液体培养基中，然后注入多孔培养皿的小孔中(每个小孔只有微量的培养基，含大约3 000个胚胎干细胞)。我们发现，原本分开的胚胎干细胞开始聚集在一起。
随后，就可以诱导这些微小的细胞聚集体，让它们全部分化为一种神经前体细胞(neural progenitor)——这类细胞通常存在于大脑前部。然后，这些细胞开始相互发送信号，经过三四天的时间，它们便自发组织成中空的球体，由单层的神经上皮细胞构成(神经上皮细胞即神经干细胞，由前体细胞分化而来)。我们把这种形成单细胞层的方法称作SFEBq培养法，即“胚状体样聚集体的快速再聚集无血清悬浮培养法”(serum-free floating culture of embryoid body-like aggregate with quick reaggregation)。
在胚胎中，神经上皮细胞接收来自细胞外的化学信号，zui终形成特异的大脑组织结构。在这些化学信号中，有一种信号可触发间脑的发育，形成视网膜和下丘脑(hypothalamus，控制食欲和其他许多基本生理功能的脑区)。成功地使胚胎干细胞形成球体之后，我们实验室开始尝试，诱导这些细胞分化成视网膜前体细胞——成熟视网膜细胞的前体。我们向SFEBq培养体系中加入了一系列蛋白质。在胚胎中，这些蛋白的作用正是诱导视网膜前体细胞的产生。

At first, we did not get much success, but in 2005, we made a technological breakthrough. In the past, when we cultured stem cells in our laboratory, the cells were only laid on the culture dish. However, in 2005, we broke through the "two-dimensional limitation" and allowed the suspension of stem cells in the culture medium. This is called "suspension culture." There are many reasons why we use this three-dimensional culture technique. First, in suspension culture, cells accumulate and form three-dimensional structures themselves, making them easier to produce complex tissues than tiled cell layers. Second, cells need to communicate with each other in order to develop complex structures , While three-dimensional c*tion is better suited to facilitate such exchanges because the cells can interact more flexibly.
Using this new method, we suspended the embryonic stem cells isolated from each other in liquid medium and injected them into the wells of a multi-well culture dish (with only a minimal amount of media per well containing approximay 3,000 embryonic stem cells). We found that originally separated embryonic stem cells began to congregate.
Subsequently, these tiny cell aggregates can be induced to differentiate them all into a neural progenitor - usually in the front of the brain. The cells then started to send signals to each other. After three or four days, they spontaneously organized into hollow spheres and consisted of a single layer of neuroepithelial cells (neural epithelial cells, neural stem cells, differentiated from precursor cells). We refer to this method of forming a single cell layer as the SFEBq culture method, that is, "serum-free floating culture of embryoid body-like aggregate with quick reaggregation" .
In embryos, neuroepithelial cells receive chemical signals from outside the cell, eventually forming specific brain tissue structures. Among these chemical signals, there is a signal that triggers the development of the diencephalon that forms the retina and the hypothalamus (the brain that controls appetite and many other basic physiological functions). After successfully bringing embryonic stem cells into the sphere, our lab started trying to induce these cells to differentiate into precursors of retinal precursor cells, mature retinal cells. We added a series of proteins to the SFEBq system. In embryos, the function of these proteins is to induce the production of retinal progenitor cells.