心肌炎麻疹病毒IgG免疫诊断试剂盒

英文名称：American FUCUS measles virus diagnostic kit

广州健仑生物科技有限公司

（广州健仑生物科技有限公司是集研制开发、销售、服务于一体的优良企业,公司产品涉及临床快速诊断试剂、食品安全检测试剂，违禁品快速检测，动物疾病防疫检测试剂，免疫诊断试剂、临床血液学和体液学检验试剂、微生物检验试剂、分子生物学检验试剂、临床生化试剂、有机试剂等众多领域，同时核心代理Panbio、FOCUS、Qiagen、IBL、CORTEZ、Fuller、Inbios、BinaxNOW、LumuQuick、日本富士、日本生研等多家有名诊断产品集团公司产品，致力于为商检单位、疾病预防控制中心、海关出入境检疫局、卫生防疫单位,缉毒系统，戒毒中心，检验检疫单位、生化企业、科研院所、医疗机构等机构与行业提供*、高品质的产品服务。此外，本公司还开展食品、卫生、环境、药品等多方面的第三方检测服务。）

主要用途：用于定量测定人血清、脑脊液或血浆中的麻疹抗体。

产品规格：96T/盒

存储条件：4-8℃

保质期：18个月

【麻疹的介绍】

麻疹病毒的*自然储存宿主是人。急性期患者是传染源，患者在出疹前6天至出疹后3天有传染性。通过飞沫传播、也可经用具、玩具或密切接触传播。麻疹传染性*，易感者接触后几乎全部发病。发病的潜伏期为9～12天。由于CD46是麻疹病毒受体，因此具有CD46的大多组织细胞均可为麻疹病毒感染的靶细胞。经呼吸道进入的病毒首先与呼吸道上皮细胞受体结合并在其中增殖，继之侵入淋巴结增殖，然后入血（在白细胞内增殖良好），形成*次病毒血症。病毒到达全身淋巴组织大量增殖再次入血，形成第二次病毒血症。此时开始发热，继之由于病毒在结膜、鼻咽粘膜和呼吸道粘膜等处增殖而出现上呼吸道卡他症状。病毒也在真皮层内增殖，口腔两颊内侧粘膜出现中心灰白、周围红色的Koplik斑，3天后出现特征性皮疹，皮疹形成的原因主要是局部产生超敏反应。一般患儿皮疹出齐24小时后，体温开始下降，呼吸道症状一周左右消退，皮疹变暗，有色素沉着。有些年幼体弱的患儿，易并发细菌性感染，如继发性支气管炎、中耳炎，尤其易患细菌性肺炎，这是麻疹患儿死亡的主要原因。大约有0.1%的患者发生脑脊髓炎，它是一种迟发型超敏反应性疾病，常于病愈1周后发生，呈典型的脱髓鞘病理学改变及明显的淋巴细胞浸润，常留有*性后遗症，病死率为15%。免疫缺陷儿童感染麻疹病毒，常无皮疹，但可发生严重致死性麻疹巨细胞肺炎。百万分之一麻疹患者在其恢复后若干年，多在学龄期前出现亚急性硬化性全脑炎（subacute sclerosing panencephalitis，SSPE）。SSPE属急性感染的迟发并发症，表现为渐进性大脑衰退，1～2年内死亡。经研究发现，患者血清及脑脊液中虽有高效价的IgG或IgM抗麻疹病毒抗体，但是用这些抗体很难分离出麻疹病毒。现认为脑组织中的病毒为麻疹缺陷病毒，由于在脑细胞内病毒M基因变异而缺乏合成麻疹病毒M蛋白的能力，从而影响病毒的装配、出芽及释放。因此，将SSPE尸检脑组织细胞与对麻疹病毒敏感细胞（如HeLa、Vero等）共同培养，可分离出麻疹病毒。

【注意事项】　　

Only the complete use of reagents to ensure that the test results, because all reagents are related, can not be mixed with other manufacturers of products. Especially standard serum, control serum and enzyme markers, must be equipped with the kit, do not use other batch products. Dilution buffer, wash solution, stop solution, and substrate solution are used for all Serun ELISA classic kits.
 
All reagents in the ELISA classic kit must be properly stored. It should be stored at 2-8 ° C in the unopened condition and used for a period of validity (see label instructions). Detailed stability and storage information will be described in detail in "8. Storage and Stability".
 
Each reagent is calibrated to ensure optimal test results. The lack of regulation or dilution of these reagents can lead to a decrease in the sensitivity of the assay.
 
Avoid exposing reagents to bright light during storage and incubation. All reagent bottle caps should be tightly closed to prevent evaporation and contamination and reagents should be free from microbial contamination as proteolytic enzyme interference will result in erroneous results.
 
Please cut the sealed bag from the mark. If the aluminum bag is damaged or the aluminum bag with the desiccant is opened but not closed by the clip, do not continue to use the microwell.
 
Allow all reagents to room temperature before starting the test.
 
When taking reagents from the reagent tube, care should be taken to use aseptic technique to prevent contamination. To avoid false-positive results, aspiration of the enzyme conjugate, the nozzle should be kept out of contact with the eyelet or sprayed on it, taking care not to mistake the cap or cap.
 
The repeatability of the test results depends on the well-mixed reagents. Shake the vial containing control serum and all diluted solution (eg using a mixer) before use.
 
Carefully pipet the reagents and strictly observe the given incubation time and temperature. Note that when the specimen / control serum, enzyme conjugate, or substrate is aspirated, the time interval between the first well and the last well sample will result in a different "pre-incubation" time, Affect the accuracy and repeatability of the measured values.
 
If the test does not strictly abide by the validity of the control quality control certification documents specific guidelines, the test results invalid.
 
Inadequate washing will affect the test results:
 
Care should be taken to wash. The washing procedure should be carried out in accordance with the instruction manual for the relevant scrubber (flat bottom hole, hole diameter 7 mm, depth 10.9 mm). It is important that all wells be filled with the same volume of wash buffer. At the end of the wash, the microplate should be back-loaded onto a paper towel and tapped gently to ensure that no wash buffer is present in all wells. Avoid bubbles! If using an automatic plate washer, pay attention to correct operation.

【检测原理】

ELISA（酶联免疫吸附测定）是涉及的免疫学过程在抗体检测的感染领域尤其得到证实。该基于抗体和抗原的特异性相互作用的检测反应。至为此目的，使用赛润ELISA classic的微量滴定板的测试条传染性病原体特异性抗原在患者样品中的结合包被的抗体存在。 其他用碱性磷酸酶标记二抗检测由此形成的免疫复合物。 该酶催化a反应过程中，无色底物对硝基苯磷酸酯在有色产物中对硝基苯酚转化。 反应产物的信号强度正比于样品中的抗体浓度用光度法检测。

心肌炎麻疹病毒IgG免疫诊断试剂盒

【试剂盒的组成】

我司同时还提供、美国FOCUS、西班牙DIA、美国trinity等试剂盒：

麻疹、风疹、甲流 、乙流、单疱疹1型、单疱疹2型、百日咳、百日咳毒素、腮腺炎、带状疱疹、单纯疱疹、HSV1型特异性、巨细胞-特异、风疹-特异、弓形虫-特异、棘球属、嗜肺军团菌、破伤风、蜱传脑炎、幽门螺旋杆菌、白色念珠菌、博氏疏螺旋体、细小病毒、钩端螺旋体、腺病毒、Q热柯克斯体、烟曲霉菌、埃可病毒、EB病毒、衣原体、耶尔森菌、空肠弯曲杆菌、炭疽杆菌、白喉、肠道病毒、柯萨奇病毒、肺炎衣原体、沙眼衣原体、土拉弗朗西斯菌、汉坦病毒、类风湿因子、呼吸道合胞病毒、单纯疱疹病毒质控品、巨细胞质控品、弓形虫质控品、风疹麻疹质控品等试剂盒以。

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The only natural storage host for measles is human. Acute phase is the source of infection, the patient 6 days before the rash to 3 days after the rash contagious. Spread through the droplets can also be used toys, toys or close contact with the spread. Highly contagious measles, susceptible contacts almost all of the disease. The incubation period of 9 to 12 days. Because CD46 is a measles virus receptor, most of the tissue cells that have CD46 can be target cells for measles virus infection. The virus that enters the respiratory tract first binds to and multiplies in the respiratory epithelial cell receptors, subsequently invades the lymph nodes and proliferates, then enters the bloodstream (proliferating well in leukocytes), forming the first viremia. The virus reached the body mass proliferation of lymphoid tissue into the blood again, the formation of the second viremia. At this point began fever, followed by the virus in the conjunctiva, nasopharyngeal mucosa and respiratory tract proliferation and other symptoms appear on the upper respiratory tract catarrhal. The virus also proliferated in the dermis. The inner mucosa of the buccal cavity appeared grayish center and surrounded by red Koplik spots. The characteristic rashes appeared after 3 days. The main cause of the rash was local hypersensitivity. General children rash out Qi 24 hours after the temperature began to decline, respiratory symptoms subsided about a week, the rash darker, pigmentation. Some young infirm children, complicated by bacterial infections, such as secondary bronchitis, otitis media, especially susceptible to bacterial pneumonia, which is the main cause of death in children with measles. About 0.1% of patients with encephalomyelitis, it is a delayed-type hypersensitivity disease, often occurs after a week of recovery, showing typical demyelinating pathology and lymphocyte infiltration, often A permanent sequelae, the case fatality rate was 15%. Children with immunodeficiency infection with measles virus, often without rash, but can occur severe lethal measles giant cell pneumonia. Measles patients one millionth of measles have subacute sclerosing panencephalitis (SSPE) more than preschool age years after their recovery. SSPE is a late-onset complication of acute infection, manifested as progressive brain failure, death within 1 to 2 years. The study found that patients with serum and cerebrospinal fluid despite high titers of IgG or IgM anti-measles virus antibodies, but with these antibodies is difficult to isolate the measles virus. It is thought that the virus in the brain tissue is a measles-deficient virus, which lacks the ability to synthesize the M protein of measles virus due to the variation of the viral M gene in the brain cells, thereby affecting the assembly, budding and release of the virus. Therefore, the SSPE autopsy brain tissue cells and measles virus-sensitive cells (such as HeLa, Vero, etc.) co-culture, measles virus can be isolated.