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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

我们很高兴发现一个重要的分子通路，ERK通路，它决定是否一个成体细胞能够重编程并帮助再生过程。操纵这一机制能促进定向治疗增强人细胞的再生可能性。
ERK通路是蛋白质从细胞表面传递信号到包含细胞遗传物质的细胞核中的一种方式。进一步研究将集中在了解这一重要途径在断肢再生中是如何调节的，以及相关过程中的其他分子。
美国斯隆-凯特琳研究所等处的研究人员创建了一种多能干细胞基因组编辑平台：iCRISPR，这一平台能快速，高效的敲除干细胞中的基因，而且还能在干细胞分化过程中，进行阶段特异性的基因敲除，这将在人类疾病复杂病理研究中大放异彩。相关文章发表于2014年6月12日的《Cell Stem Cell》杂志上。
人体多能干细胞(hPSCs)不仅能被用于临床的再生研究应用中，而且也能作为解析复杂性状和特征的*平台，阐明其背后的基因和分子途径。为了实现这一目的，科学家们开发了多种遗传操控方法，但是这些方法依然存在各种问题，我们需要快速，具有可操控性的生物学手段。
在这篇文章中，研究人员就利用CRISPR和TALEN，这两种备受关注的基因组编辑技术，研发出了一种人类多能干细胞基因组编辑平台。研究人员将这一平台称为iCRISPR。
iCRISPR能用于基因功能丧失研究中，快速，高效的敲除人体多能干细胞中的等位基因，也可以针对一些精确的疾病模型，通过特定的核苷酸变换，进行多能干细胞纯合体敲除。
通过进一步实验，研究人员验证了双重和三重基因敲除hPSC细胞系一步法的有效性，同时也证明了在多能干细胞分化过程中能进行阶段特异性诱导基因敲除，这对于发育生物学研究来说意义重大。
由此研究人员指出，iCRISPR平台尤其适合用于解析人类疾病研究中的复杂遗传相互作用，以及多效性基因功能，这将有助于进行人体多能干细胞高通量遗传分析。

We are happy to find an important molecular pathway, the ERK pathway, that determines whether an adult cell can reprogram and help regenerate the process. Manipulating this mechanism can promote targeted therapy to enhance the regenerative potential of human cells.
The ERK pathway is one way in which proteins transfer signals from the cell surface into the nucleus containing cytogenetic material. Further research will focus on understanding how this important pathway is regulated in limb amputation and other molecules involved in the process.
Researchers at the U.S.-based Sloan-Caitlin Institute have created iCRISPR, a pluripotent stem cell genomics editing platform that rapidly and efficiently knocks out genes in stem cells and helps them differentiate stem cells during stem cell differentiation, Performing stage-specific knockouts will shine in the complex pathology of human disease. The article appeared in the June 12, 2014 issue of Cell Stem Cell.
Not only can human pluripotent stem cells (hPSCs) be used in clinical regenerative research applications, but also as a unique platform for analyzing complex traits and traits, elucidating the underlying genetic and molecular pathways. To achieve this goal, scientists have developed a variety of genetic manipulation methods, but there are still a variety of problems with these methods, and we need fast, manipulative biological tools.
In this article, researchers developed a human pluripotent stem cell genome editing platform using two well-regarded genome editing technologies, CRISPR and TALEN. The researchers call this platform iCRISPR.
iCRISPR can be used in gene loss studies to rapidly and efficiently knock out alleles in pluripotent human pluripotent stem cells and to target specific models of disease through specific nucleotide transforms for pluripotent stem cell homozygous knocking except.
Through further experiments, the researchers validated the one-step efficacy of double and triple knockout hPSC cell lines, and also demonstrated that stage-specific induction of gene knockdown during pluripotent stem cell differentiation can be performed in developmental biology Meaningful.
As a result, the researchers noted that the iCRISPR platform is particularly well-suited for the analysis of complex genetic interactions in human disease research and pleiotropic gene functions that will facilitate high-throughput genetic analysis of human pluripotent stem cells.