- 产品描述
甲型流感病毒H10亚型核酸检测试剂盒
广州健仑生物科技有限公司
广州健仑长期供应各种PCR试剂盒,主要代理进口和国产品牌的流行病毒PCR检测试剂盒。例如:甲乙型流感病毒核酸检测试剂盒、黄热病毒核酸检测试剂盒、诺如病毒核酸检测试剂盒、登革病毒核酸检测试剂盒、基孔肯雅病毒核酸检测试剂盒、结核杆菌核酸病毒检测试剂盒、孢疹病毒核算检测试剂盒、西尼罗河病毒PCR检测试剂盒、呼吸道合胞病毒核酸检测试剂盒、冠状病毒PCR检测试剂盒等等。虫媒体染病系列、呼吸道病原体系列、发热伴出疹系列、消化道及食源感染系列。
广州健仑长期供应各种流感检测试剂,包括进口和国产的品牌,主要包括日本富士瑞必欧、日本生研、美国BD、美国NovaBios、美国binaxNOW、凯必利、广州创仑等主流品牌。
甲型流感病毒H10亚型核酸检测试剂盒
【产品说明书】
【包装规格】大包装,24 反应/盒;大包装,48 反应/盒
【预期用途】
本试剂盒适用于检测黄热病病毒所致的急性传染病的快速初筛检测,结合病毒的分离鉴定,对快速检测黄热病病原有很重要的指导意义。检测结果仅供研究,不用于临床诊断。
【检验原理】
本试剂盒基于实时荧光PCR技术,选取黄热病病毒核酸片断一个相对保守区作为扩增靶区域,设计特异性引物及荧光探针,通过一步法实时荧光 PCR 体系扩增对黄热病病毒进行定性检测。反应体系中除一对特异性引物和一条特异性荧光探针外,还配以对应的PCR反应 Buffer、逆转录酶、热启动Taq酶、核苷酸单体(dNTPs)、Mg2+等成分,可实现对黄热病病毒核酸灵敏特异地检测。
【储存条件及有效期】
试剂盒保存于-20±5℃,有效期 9 个月。
试剂盒在 37℃可保存72h;试剂在 4℃可保存7天;避免反复冻融,反复冻融次数不超过 7 次;试剂开瓶次数不超过 7 次。运输采用干冰保持低温,运输时间不应超过 3 天。试剂盒生产日期见产品标签。
【适用仪器】
包括 ABI7500、ABI7300、LightCycler480 等荧光定量 PCR 仪。
【样本要求】
1.适用样品类型:血清
2.样品采集(注意无菌操作)
3.样品保存和运送
人血清标本装入螺口塑料血清管,用耐低温油性记号笔记上编号,于-70℃以下运输或保存待检。标本处理和 RNA 提取亦可参照有关国家标准或有关行业标准中推荐的方法。
【检测方法】
1.样本处理和核酸提取(样本处理区)
2.PCR 试剂准备(试剂准备区)
3.加样(样本制备区)
3.1 ABI Prism 7500 仪器设置(ABI 7500 仪器操作为例,ABI 7300 仪器参照此操作及仪器操作手册)
3.2 LightCycler480 仪器设置
4. 结果分析
4.1 反应结束后保存检测数据文件。
4.2 分析条件设置:根据分析后图像调节 Baseline 的 start 值、stop 值以及 Threshold 的 Value 值(用户可根据实际情况自行调整, Start 值可以在 3~15、End 值可设在 5~20,调整阴性对照的扩增曲线平直或低于阈值线),点击 Analysis 自动获得分析结果,在 Report 界面察看结果。
【质量控制】
阴性质控品:FAM 检测通道无明显扩增曲线;
阳性质控品:FAM 检测通道有明显扩增曲线,Ct 值≤32;以上要求需在同一次实验中同时满足,否则,本次实验无效,需重新进行。
【结果判定】
- 如果检测样品无扩增曲线或 Ct 值>38,可判样品为黄热病病毒阴性;
- 如果检测样品 Ct 值≤38,且曲线有明显的扩增曲线,可判样品为黄热病病毒阳性;
【检测方法的局限性】
样本检测结果和样本收集、处理、运送及保存质量有关,其中任何失误都将会导致假阴性结果。如果样本处理时没有控制好交叉污染,可能出现假阳性结果。
我司还提供其它进口或国产试剂盒:登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。
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【公司名称】 广州健仑生物科技有限公司
【市场部】 杨永汉
【】
【腾讯 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室
胸膜疾病
包括大量胸腔积液、气胸,胸膜增厚。此类疾病突出的临床表现是与体位无关的呼吸困难,体格检查示气管可移位,胸腔受累部位肋间隙可增宽或变窄,叩诊呈浊音或鼓音,肺功能检查为限制性肺通气功能障碍,胸片、肺CT检查有胸腔积液、积气或胸膜增厚的表现。
急性肺栓塞
是由于内源性或外源性栓子堵塞肺动脉或其分支引起肺循环障碍的临床综合征。有两个基本临床症候群:肺梗死:突发呼吸困难、胸痛、咯血、胸膜摩擦音,不能解释的呼吸困难。该病有引起栓子形成或高凝状态的基础疾病,胸片显示肺出现卵圆形或三角形浸润细菌影,重症者肺动脉段突出,实验室检查可见血D-二聚体水平升高而肌钙蛋白水平不高,心电图可有异常,心、肺动脉造影及放射性核素肺灌注扫描可准确了解栓塞所在的部位及范围。
周围循环缺血性疾病
常见于各种原因引起的严重休克,由于周围血管收缩心排血量减少,循环血容量不足,或周围动脉缩窄,周围组织血流灌注不足,缺氧致皮肤黏膜呈青紫色。新生儿严重腹泻,如果治疗不当,由于脱水,亦可造成紫绀。再者,雷诺病、肢端发绀症亦属此类。雷诺病的特点是双手或双足有麻木或厥冷感,发作时各指(趾)出现典型的皮肤苍白-青紫-潮红的雷诺现象。手足紫绀症的特点主要是在常温下持续的毛细血管前小动脉痉挛,导致血流减少,皮肤青紫和皮温降低,红外热成像荧屏为暗黑色细菌影或不显影。
周围循环淤血性疾病
如右侧心力衰竭、缩窄性心包炎以及局部静脉病变(血栓性静脉炎、上腔静脉综合征、下肢静脉曲张),其发生紫绀的机制是体循环淤血,周围血流缓慢,氧在周围组织被摄取过多所致。
造成紫绀的疾病非常多,上述只是列举了一些常见的疾病,尚有很多并不常见的但也可导致紫绀的疾病,比如红细胞增多症、膈疝等均可引起紫绀。年龄较小的患者,突然出现紫绀,伴有吸气时呼吸困难,刺激性干咳,多为呼吸道异物;如出生后或幼年时出现紫绀,且颜色较深,呈暗紫色,发现手指、脚趾*增粗如杵头状,多为发绀型先天性心脏病;如皮肤粘膜呈灰色,发生于四肢末端,细菌或轻微加温则减轻或消失,伴有昏迷,目合口张,手撒不握,面色苍白,二便失禁,多见于休克病人;如轻度紫绀,持续时间较长,在肺部感染和劳累后颜色加深,则多为心肺疾患。
由于紫绀病情较重,病因又极其复杂,故一旦发现,应立即到医院就诊,及早治疗,以免延误时机。
Pleural disease
Including pleural effusion, pneumothorax, pleural thickening. Outstanding clinical manifestations of these diseases are unrelated to the position of dyspnea, physical examination showed the trachea can be displaced, the chest parts of the intercostal space can be widened or narrowed, percussion was voiced or drum sound, pulmonary function tests for restrictive lung ventilation Dysfunction, chest radiography, chest CT examination of pleural effusion, pneumatosis or pleural thickening performance.
Acute pulmonary embolism
Is due to endogenous or exogenous emboli plug the pulmonary artery or its branches cause clinical symptoms of pulmonary circulatory disorders. There are two basic clinical syndromes: pulmonary infarction: sudden breathing problems, chest pain, hemoptysis, pleural friction sounds, unexplained dyspnea. The disease has caused the formation of emboli or hypercoagulable state of the underlying diseases, chest X-ray showed oval or triangle infiltration of bacteria, severe pulmonary artery prominent, laboratory examination showed increased levels of D-dimer and muscle Calprotectin level is not high, the ECG may be abnormal, heart, pulmonary angiography and radionuclide lung perfusion scan can accuray understand the location and scope of embolism.
Circulatory ischemia around the disease
Common in a variety of causes of severe shock, due to peripheral vasoconstriction cardiac output decreased circulatory insufficiency, or narrowing of peripheral arteries, inadequate perfusion of peripheral tissue, hypoxia to the skin and mucous membranes were purple. Severe neonatal diarrhea, if not treated properly, due to dehydration, can also cause cyanosis. In addition, Raynaud's disease, acromegaly also belong to this category. Raynaud's disease is characterized by numbness or convulsions in both hands or feet, typical pale skin-bruising-flushing Renault at each finger (toe) at onset. Hand, foot and cyanosis is characterized by persistent pre-capillary arteriolar spasm at room temperature, leading to reduced blood flow, skin bruising and skin temperature decreased, infrared thermal imaging screen dark-black bacteria or no shadow.
Circulatory congestion around the disease
Such as right heart failure, constrictive pericarditis and local venous lesions (thrombophlebitis, superior vena cava syndrome, varicose veins), its mechanism of cyanosis occurs systemic congestion, peripheral blood flow is slow, oxygen in the surrounding tissue Was taken too much.
Cyanosis caused by very many, the above is just a list of some common diseases, there are many are not common but can also lead to cyanosis diseases, such as polycythemia, diaphragmatic hernia can cause cyanosis. Older patients, sudden cyanosis, accompanied by breathing difficulties in breathing, irritating dry cough, mostly foreign body particles; such as cyanosis after birth or juvenile, and the darker, dark purple, finger, toe tip increased Coarse, such as pestle-shaped head, mostly cyanotic congenital heart disease; such as skin mucosa was gray, occurred in the extremities, bacteria or mild warming is to reduce or disappear, with a coma, head and mouth Zhang, hand Caesar, face Pale, second incontinence, more common in patients with shock; such as mild cyanosis, longer duration, darker after infection and exertion in the lungs, are mostly heart and lung disease.
Due to severe cyanosis, the cause is extremely complex, so once found, you should immediay go to the hospital for treatment, early treatment, so as not to delay the opportunity.