甲型流感病毒IgG基因捕获法检测试剂盒

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甲型流感病毒IgG基因捕获法检测试剂盒

基因捕获法是利用随机插入突变进行基因敲除的新型方法。通常基因捕获载体还包括一个无启动子的报道基因，通常是neo 基因，neo 基因插入到ES 细胞染色体组中，并利用捕获基因的转录调控元件实现表达的ES 克隆可以很容易地在含G418 的选择培养基中筛选出来，从理论上讲，在选择培养基中存活的克隆应该100%地含有中靶基因。中靶基因的信息可以通过筛选标记基因侧翼cDNA或染色体组序列分析来获得。

基因捕获法是利用随机插入突变进行基因敲除的新型方法。通常基因捕获载体还包括一个无启动子的报道基因，通常是neo 基因，neo 基因插入到ES 细胞染色体组中，并利用捕获基因的转录调控元件实现表达的ES 克隆可以很容易地在含G418 的选择培养基中筛选出来，从理论上讲，在选择培养基中存活的克隆应该100%地含有中靶基因。中靶基因的信息可以通过筛选标记基因侧翼cDNA或染色体组序列分析来获得。

用常规方法进行基因敲除研究需耗费大量的时间和人力，研究者必须针对靶位点在染色体组文库中筛选相关的染色体组克隆，绘制相应的物理图谱，构建特异性的基因敲除载体以及筛选中靶ES 细胞等，通常一个基因剔除纯合子小鼠的获得需要一年或更长的时间。面对人类基因组计划产生出来的巨大的功能未知的遗传信息，传统的基因敲除方法显得有些力不从心。因此，基因捕获法应运而生，利用基因捕获可以建立一个携带随机插入突变的ES 细胞库，节省大量筛选染色体组文库以及构建特异打靶载体的工作及费用，更有效和更迅速地进行小鼠染色体组的功能分析。
此方法的缺点是只能剔除在Es 细胞中表达的基因．单种的细胞类型中表达的基因数目约为I04，现在的基因捕获载体从理论上来讲应能剔除所有在ES细胞表达的基因，因此，在ES 细胞中进行基因捕获还是大有可为的。用基因捕获法进行基因剔除的另一个缺点是无法对基因进行精细的遗传修饰。
 

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

Gene capture is a novel method of gene knockout using random insertions. Generally, the gene capture vector also includes a promoterless reporter gene, usually a neo gene. The neo gene is inserted into the genome of the ES cell and an ES clone expressing the transcriptional regulatory element of the capture gene can be easily expressed in a G418-containing The selection medium is screened, and theoretically, the clone that survives in the selection medium should contain 100% of the target gene. The target gene information can be obtained by screening the flanking cDNA or genomic sequence analysis of the marker gene.

Gene capture is a novel method of gene knockout using random insertions. Generally, the gene capture vector also includes a promoterless reporter gene, usually a neo gene. The neo gene is inserted into the genome of the ES cell and an ES clone expressing the transcriptional regulatory element of the capture gene can be easily expressed in a G418-containing The selection medium is screened, and theoretically, the clone that survives in the selection medium should contain 100% of the target gene. The target gene information can be obtained by screening the flanking cDNA or genomic sequence analysis of the marker gene.

Gene knockout studies using conventional methods take a considerable amount of time and manpower. Researchers have to screen for the relevant genomic clones in the genomic library against the target site, map the corresponding physical maps, construct specific knockout vectors, and Screening of target ES cells and the like usually takes one year or more to obtain a gene knockout homozygous mouse. In the face of huge untapped genetic information generated by the Human Genome Project, traditional methods of gene knock-outs seem a bit powerless. Therefore, the gene capture method came into being, the use of gene capture can create a library of ES cells carrying random insertion mutations, saving a large number of screening of the genome library and the construction of specific targeting vector work and costs more efficient and more prompt mouse chromosome Group functional analysis.
The disadvantage of this method is that only the genes expressed in Es cells can be excluded. The number of genes expressed in a single cell type is about 104, and now the gene capture vector is theoretically able to knock out all the genes expressed in ES cells. Therefore, gene capture in ES cells is still promising. Another drawback of gene knockout using gene trapping is the inability to fine-tune genes for genetic modification.