乙型流感诊断试剂盒（快检方法）

广州健仑生物科技有限公司

广州健仑长期供应各种流感检测试剂，包括进口和国产的品牌，主要包括日本富士瑞必欧、日本生研、美国BD、美国NovaBios、美国binaxNOW、凯必利、广州创仑等主流品牌。

乙型流感诊断试剂盒（快检方法）

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

实验设备
1. 电泳设备
2. 紫外灯
实验步骤
1．电泳槽用0.3%H2O2浸泡30min，DEPC水冲洗晾干。 
2．铺胶（40ml） 
       琼脂糖                0.55g   
       DEPC水             36ml 
      10×MOPS         5ml 
      37%甲醛            9ml 
称0.55克琼脂糖加36ml DEPC水，微波炉加热溶解琼脂糖，肉眼观察无颗粒状悬浮物。冷却至50-60℃，加9ml 甲醛（37%）和5ml 10×电泳缓冲液，然后灌制凝胶，插入合适长度和宽度的梳子，于室温放置30分钟以上使凝胶凝固。
3．RNA样品处理（以一条泳道为例）一般取0.3 g的总RNA，加入1/5体积的5×加样缓冲液，65℃加热5 min，冰上骤冷，以消除RNA的二级结构。建议上样前在RNA样品中加入0.5-1.0 ul的溴化乙锭（EB，浓度1.0 mg/mL），而不在胶中加EB，这样电泳后的背景较低。配好的甲醛变性胶先在1×甲醛变性胶电泳缓冲液中预电泳15min。RNA样品在5-10 V/cm的电压降下电泳30min。
4．加2.0ml甲醛凝胶加样缓冲液（10×） 
5．加样和电泳 
将凝胶预电泳15min，电压降为5-10 v/cm。随后加样品和标准物，以3-4v/cm电压降电泳，电泳液为1×MOPS电泳缓冲液。直至溴酚蓝迁移至胶下游的3/4处。

Laboratory equipment
Electrophoresis equipment
2. UV lamp
Experimental steps
1. Electrophoresis tank with 0.3% H2O2 soak 30min, DEPC water rinse to dry.
2. Shop plastic (40ml)
       Sepharose 0.55g
       DEPC water 36ml
      10 × MOPS 5ml
      37% formaldehyde 9ml
Weigh 0.55 grams of agarose and 36 ml of DEPC water, dissolve the agarose in a microwave oven, and visually observe the non-granular suspension. Cool to 50-60 ° C. Add 9ml of formaldehyde (37%) and 5ml of 10x running buffer then gel and insert a comb of appropriate length and width for 30 minutes at room temperature to allow the gel to set.
3. RNA sample processing (taking one lane as an example) Generally, take 0.3 g of total RNA, add 1/5 volume of 5 × loading buffer, heat at 65 ° C for 5 min and quench on ice to eliminate RNA secondary structure. It is recommended that 0.5-1.0 μl of ethidium bromide (EB, 1.0 mg / mL) be added to the RNA sample prior to loading without adding EB to the gel so that the background after electrophoresis is low. Formaldehyde denatured plastic first in 1 × formalin gel electrophoresis buffer pre-electrophoresis 15min. RNA samples were electrophoresed for 30 min at a voltage drop of 5-10 V / cm.
4. Add 2.0ml formaldehyde gel loading buffer (10 ×)
5. Sample and electrophoresis
The gel pre-electrophoresis 15min, the voltage drop to 5-10 v / cm. Then add samples and standards, with 3-4v / cm voltage drop electrophoresis, electrophoresis solution 1 * MOPS electrophoresis buffer. Until bromophenol blue migrates to 3/4 of the gel downstream.