甲型乙型流感诊断试剂盒（快检方法）

广州健仑生物科技有限公司

广州健仑长期供应各种流感检测试剂，包括进口和国产的品牌，主要包括日本富士瑞必欧、日本生研、美国BD、美国NovaBios、美国binaxNOW、凯必利、广州创仑等主流品牌。

甲型乙型流感诊断试剂盒（快检方法）

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

二甲苯
酒精（100％，95％）
EDTA抗原修复工作液（pH8.0，稀释50倍使用）
0.5M Tris-NaCl (TBS) pH7.4（稀释10倍使用）
DAB显色液（临用前配制：试剂盒A、B、C各50ul，于1ml水中混匀）。
方法
1.  石蜡切片置60℃烘箱中烘烤过夜
2.  二甲苯中脱蜡，梯度酒精入水(无水乙醇，95％酒精)，浸泡于蒸馏水中待用
3.  抗原修复
取500mlEDTA抗原修复工作液于1000ml烧杯中，在小功率电炉上加热，至似沸微沸（为了防止脱片）。将组织切片缓慢放入烧杯。继续加热，保持液体在微沸状态20分钟。将烧杯移开火源，室温下自然冷却后取出切片，蒸馏水洗1次3分钟，TBS洗2次每次3分钟。
4.  每张切片加一滴或50ul 3％过氧化氢溶液，室温下孵育10min， 以阻断内源性过氧化物酶的活性。 TBS冲洗3次，每次3min。
5.  除去TBS液，加一滴或50ul 一抗（灭活PLB免疫小鼠所得到的多抗，1:3000稀释），阴性对照采用普通血清， 室温下孵育2小时，或4℃过夜。
6.  TBS洗3次，每次5min，除去TBS液，每张切片加一滴或50ul polymer enhancer（试剂A）， 室温下孵育20min， TBS冲洗3次， 每次3min。
7.  除去TBS液，每张切片加一滴或50ul 过氧化物酶标记的抗鼠/兔聚合物（试剂B），室温下孵育30min，TBS洗3次，每次3min。
8.  除去TBS液，每张切片加一滴或50ul 新鲜配制的DAB，显色3－10min。
9.  自来水冲洗，苏木素复染10min，水冲洗。0.1%的盐酸分化，自来水冲洗，TBS返蓝。
10.  不需脱水，直接用中性树脂封片。

Xylene
Alcohol (100%, 95%)
EDTA antigen repair working solution (pH8.0, diluted 50 times use)
0.5M Tris-NaCl (TBS) pH7.4 (diluted 10 times used)
DAB color reagent (before preparation: kit A, B, C of each 50ul, 1ml water mix).
method
1. Paraffin slices were oven-baked overnight at 60 ° C
2. Xylene dewaxing, gradient alcohol into the water (ethanol, 95% alcohol), soaked in distilled water to be used
Antigen repair
Take 500mlEDTA antigen repair working solution in 1000ml beaker, heated in a low-power electric furnace, to the boiling-like micro boiling (in order to prevent peeling). Slowly place tissue sections into beakers. Continue to heat, keep the liquid in a slightly boiling state for 20 minutes. The beaker is removed from the source of fire, cooled at room temperature and then remove the slice, washed with distilled water 3 minutes 3 times, TBS wash 2 times each 3 minutes.
4. Add one drop or 50μl of 3% hydrogen peroxide solution to each slice and incubate at room temperature for 10min to block the activity of endogenous peroxidase. TBS rinse 3 times, each 3min.
5. Remove the TBS solution and add one drop or 50 ul of primary antibody (1: 3000 dilution of polyclonal antibody obtained from mice immunized with PLB). The negative control was incubated with normal serum for 2 hours at room temperature or 4 ° C overnight.
6. TBS wash three times, each time 5min, remove the TBS solution, add one drop or 50ul of polymer enhancer per slice (Reagent A), incubate 20min at room temperature, TBS rinse 3 times, each 3min.
7. Remove the TBS solution by adding one drop or 50ul of peroxidase-labeled anti-mouse / rabbit polymer (Reagent B) to each section. Incubate for 30 min at room temperature and 3x TBS for 3 min each.
8. Remove the TBS solution, add one drop or 50ul of freshly prepared DAB per slice, and color develop 3-10min.
9. Tap water, hematoxylin counterstain 10min, water rinse. 0.1% hydrochloric acid differentiation, tap water rinse, TBS return blue.
10 without dehydration, direct neutral resin seal.