流感B病毒诊断试剂盒（快检方法）

广州健仑生物科技有限公司

广州健仑长期供应各种流感检测试剂，包括进口和国产的品牌，主要包括日本富士瑞必欧、日本生研、美国BD、美国NovaBios、美国binaxNOW、凯必利、广州创仑等主流品牌。

流感B病毒诊断试剂盒（快检方法）

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

操作步骤
1. 标准品的稀释与加样：在酶标包被板上设标准品孔10孔，在*、第二孔中分别加标准品100μl，然后在*、第二孔中加标准品稀释液50μl，混匀；然后从*孔、第二孔中各取100μl分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50μl，混匀；然后在第三孔和第四孔中先各取50μl弃掉，再各取50μl分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul，混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50μl，混匀后从第七、第八孔中分别取50μl加到第九、第十孔中，再在第九第十孔分别加标准品稀释液50μl，混匀后从第九第十孔中各取50μl弃掉。（稀释后各孔加样量都为50μl，浓度分别为36ng/L，24 ng/L ，12 ng/L，6ng/L，3 ng/L）。
2. 加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品zui终稀释度为5倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。
3. 温育：用封板膜封板后置37℃温育30分钟。
4. 配液：将20倍浓缩洗涤液用蒸馏水20倍稀释后备用。
5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复5次，拍干。

Steps
1. Standard dilution and sample loading: Prepare standard wells 10 wells in the enzyme-coated plate, add 100 μl each to the first and second wells, and then add standards to the first and second wells Then add 100μl from the first well and the second well to the third well and the fourth well, add 50μl standard diluent to the third and the fourth wells, and mix well; Then take out 50μl in each of the third and fourth wells, discard 50μl each and add them to the fifth and the sixth wells, respectively, and then add 50μl standard diluent to the fifth and the sixth wells, respectively; After mixing, take 50μl from each of the fifth and sixth wells and add them to the seventh and the eighth wells respectively, then add 50μl standard diluent in the seventh and the eighth wells respectively, Eight holes were added 50μl added to the ninth and tenth holes, and then in the ninth tenth hole standard diluent were added 50μl, after mixing from the ninth tenth hole each take 50μl discarded. (Each well was diluted to 50 μl at a dilution of 36 ng / L, 24 ng / L, 12 ng / L, 6 ng / L, 3 ng / L).
2. Sample loading: Set blank holes (blank control wells without sample and enzyme reagent, the rest of the same operation), the sample hole. Add 40μl sample diluent to the sample well on the ELISA plate, then add 10μl test sample (5 times final sample dilution). Add samples will be added to the bottom of the ELISA plate hole, try not touch the hole wall, gently shaking to mix.
3. Incubation: Seal the plate with a sealing plate and incubate at 37 ° C for 30 minutes.
4. Dosage: 20 times concentrated cleaning solution diluted 20 times with distilled water reserve.
5. Wash: Carefully remove the sealing plate membrane, discard the liquid, dry it, fill each well with a washing solution, and let it stand for 30 seconds before it is discarded. Repeat 5 times and pat dry.