芬兰 流感病毒抗原检测试剂盒（胶体金法）

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芬兰 流感病毒抗原检测试剂盒（胶体金法）

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

一，免疫组织化学简介
免疫组织化学又称免疫细胞化学，是指带显色剂标记的特异性抗体在组织细胞原位通过抗原抗体反应和组织化学的呈色反应，对相应炕原进行定性、定位、定量测定的一项新技术。它把免疫反应的特异性、组织化学的可见性巧妙地结合起来，借助显微镜(包括荧光显微镜、电子显微镜)的显像和放大作用，在细胞、亚细胞水平检测各种抗原物质(如蛋白质、多肽、酶、激素、病原体以及受体等)。
二，免疫组化技术的基本原理
免疫组化技术是一种综合定性、定位和定量；形态、机能和代谢密切结合为一体的研究和检测技术。在原位检测出病原的同时，还能观察到组织病变与该病原的关系，确认受染细胞类型，从而有助于了解疾病的发病机理和病理过程。
免疫酶组化技术是通过共价键将酶连接在抗体上，制成酶标抗体，再借酶对底物的特异催化作用，生成有色的不溶性产物或具有一定电子密度的颗粒，于普通显微镜或电镜下进行细胞表面及细胞内各种抗原成分的定位，根据酶标记的部位可将其分为直接法（一步法）、间接法（二步法）、桥联法（多步法）等，用于标记的抗体可以是用免疫动物制备的多克隆抗体或特异性单克隆抗体，是特异性强的高效价的单克隆抗体。直接法是将酶直接标记在*抗体上，间接法是将酶标记在第二抗体上，检测组织细胞内的特定抗原物质。目前通常选用免疫酶组化间接染色法。

First, the introduction of immunohistochemistry
Immunohistochemistry, also known as immunocytochemistry, refers to the specific reagent with a reagent labeled with a chromogenic reagent in situ through the antigen-antibody reaction and histochemical color reaction, the corresponding kang original qualitative, localization, quantitative determination of a New technology. It cleverly combines the specificity of immunological reactions and the visibility of histochemistry to detect the presence of various antigenic substances (such as proteins, proteins, and proteins) at the cellular and subcellular level through the imaging and amplification of microscopy (including fluorescence microscopy and electron microscopy) Polypeptides, enzymes, hormones, pathogens and receptors, etc.).
Second, the basic principles of immunohistochemistry
Immunohistochemistry is a comprehensive qualitative, positioning and quantitative; morphology, function and metabolism as one of the closely integrated research and testing technology. In situ detection of the pathogen at the same time, but also observed the relationship between tissue lesions and the pathogen to confirm the type of infected cells, which helps to understand the pathogenesis of the disease and pathological processes.
Enzyme immunohistochemistry is a covalent bond to the enzyme linked to the antibody, made of enzyme-labeled antibody, and then by the enzyme on the substrate for specific catalysis to generate colored insoluble products or have a certain electron density of particles in the ordinary microscope Or electron microscopy of cell surface and intracellular antigen components of the location, according to the site of enzyme markers can be divided into direct method (one-step method), indirect method (two-step method), bridge method (multi-step method) The antibody used for labeling may be a polyclonal antibody or a specific monoclonal antibody prepared by immunizing an animal, preferably a highly specific, highly titer monoclonal antibody. The direct method is to label the enzyme directly on the primary antibody, the indirect method is to label the enzyme on the secondary antibody and detect the specific antigenic substance in the tissue cells. At present, immunoenzymatic staining is often used indirect staining.