流感病毒抗原检测试剂盒（免疫层析法）

广州健仑生物科技有限公司

广州健仑长期供应各种流感检测试剂，包括进口和国产的品牌，主要包括日本富士瑞必欧、日本生研、美国BD、美国NovaBios、美国binaxNOW、凯必利、广州创仑等主流品牌。

流感病毒抗原检测试剂盒（免疫层析法）

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

欢迎咨询

欢迎咨询2042552662

想了解更多的产品及服务请扫描下方二维码：

【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

【】 
【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

三，免疫组化步骤
1，  切片，烤片60℃，1h；
2，脱蜡及复水
   二甲苯10min，二甲苯10min，100％乙醇5min，95％乙醇5min，90％乙醇5min，85％乙醇5min，80％乙醇5min， 75％乙醇5min，60％乙醇5min，50％乙醇5min，30％乙醇5min，自来水1min，双氧水1min；
3，1份30％H2O2加10份蒸馏水，室温10min，蒸馏水洗3次，每次3min；
4，微波修复
将切片浸入0.01M枸橼酸缓冲液，微波中zui大火力（98℃-100℃）加热至沸腾，冷却（约5-10min），反复两次；
5，将切片自然冷却至室温，PBS洗涤3次，每次5min；
6，封闭，5％BSA，室温20min，甩去多余液体；
7，滴加一抗，37℃，1h，或者4℃过夜；
8，PBS洗涤3次，每次3min；
9，滴加二抗，37℃，15-30min；
10，PBS洗涤3次，每次3min；
11，滴加SABC，37℃， 30min；
12，PBS洗涤3次，每次5min；
13，1ml蒸馏水中分别滴加显色剂，混匀；
14，DAB显色剂配置好后，滴加于切片，室温，镜下检测反应时间（约5min）；
15，自来水冲洗干净，过蒸馏水；
16，苏木素复染2min，自来水冲洗；
17，脱水
30％乙醇3min，50％乙醇3min，70％乙醇3min，80％乙醇3min，90％乙醇3min，95％乙醇3min，100％乙醇3min，二甲苯20min；
18，树胶封片，镜检。

Third, immunohistochemistry steps
1, sliced, baked slices 60 ℃, 1h;
2, dewaxing and rehydration
   10% xylene 10min 100% ethanol 5min 95% ethanol 5min 90% ethanol 5min 85% ethanol 5min 80% ethanol 5min 75% ethanol 5min 60% ethanol 5min 50% ethanol 5min 30% Ethanol 5min, tap water 1min, hydrogen peroxide 1min;
3,1 30% H2O2 plus 10 parts of distilled water, room temperature 10min, distilled water 3 times, each 3min;
4, microwave repair
The slices were immersed in 0.01M citrate buffer, the maximum heat in the microwave (98 ℃ -100 ℃) was heated to boiling, cooling (about 5-10min), repeated twice;
5, the sections were cooled to room temperature, washed with PBS 3 times, each 5min;
6, closed, 5% BSA, room temperature 20min, throw off excess liquid;
7, dropping a primary antibody, 37 ℃, 1h, or overnight at 4 ℃;
8, washed with PBS three times, each time 3min;
9, dropping secondary antibody, 37 ℃, 15-30min;
10, PBS washed 3 times, each 3min;
11, dropping SABC, 37 ℃, 30min;
12, PBS washed 3 times, each 5min;
13,1 ml of distilled water were dropped reagent, mixing;
14, DAB reagent configuration, dropping in slices, room temperature, microscopic detection reaction time (about 5min);
15, tap water rinse, distilled water;
16, hematoxylin counterstain 2min, tap water rinse;
17, dehydration
30% ethanol 3min, 50% ethanol 3min, 70% ethanol 3min, 80% ethanol 3min, 90% ethanol 3min, 95% ethanol 3min, 100% ethanol 3min,
18, gum seal, microscopic examination.