乙型流感病毒抗原检测试剂盒（免疫层析法）

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乙型流感病毒抗原检测试剂盒（免疫层析法）

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3，产生组织切片非特异性染色
1）抗体孵育时间过长、抗体浓度高易增加背景着色。这可通过缩短一抗/二抗孵育时间、稀释抗体来控制。这是zui重要的一条；
2）一抗用多克隆抗体易出现非特异性着色，建议试用单克隆抗体看看；
3）内源性过氧化物酶和生物素在肝脏、肾脏等组织含量很高（含血细胞多的组织），需要通过延长灭活时间和增加灭活剂浓度来降低背景染色；
4）非特异性组分与抗体结合，这需要通过延长二抗来源的动物免疫血清封闭时间和适当增加浓度来加强封闭效果；
5）DAB孵育时间过长或浓度过高；
6）PBS冲洗不充分，残留抗体结果增强着色，在一抗、二抗或SP孵育之后的浸洗尤为重要；
7）标本染色过程中经常出现干片，这容易增强非特异性着色。
4，免疫组化染色呈阴性结果
1）抗体浓度和质量问题以及抗体来源选择错误；
2）抗原修复不全，对于甲醛固定的组织必须用充分抗原修复来打开抗原表位，以利于与抗体结合;建议微波修复用高火4次*6min试试。有人做过实验，这是*的时间和次数。若不行，还可高压修复；
3）组织切片本身这种抗原含量低；
4）血清封闭时间过长；
5）DAB孵育时间过短；
6）细胞通透不全，抗体未能充分进入胞内参与反应；
7）开始做免疫组化，我建议你一定要首先做个阳性对照片，排除抗体等外的方法问题。

3, resulting in non-specific tissue section staining
1) antibody incubation time is too long, high antibody concentration easily increase background coloring. This can be controlled by reducing the primary antibody / secondary antibody incubation time and diluting the antibody. This is the most important one
2) The first antibody polyclonal antibody prone to non-specific coloring, it is recommended to try monoclonal antibody to see;
3) endogenous peroxidase and biotin in the liver, kidneys and other tissues is very high (including blood cells and more tissue), need to extend the inactivation time and increase the concentration of inactivator to reduce the background staining;
4) non-specific components and antibodies, which need to extend the secondary antibody from the immune serum to close time and the appropriate increase in concentration to enhance the sealing effect;
5) DAB incubation time is too long or too high concentration;
6) Inadequate washing with PBS, enhanced staining with residual antibody results, and dipping after primary antibody, secondary antibody or SP incubation are particularly important;
7) Dry specimens often appear during specimen dyeing, which easily enhances nonspecific staining.
4, immunohistochemistry showed negative results
1) Antibody concentration and quality problems and wrong selection of antibody sources;
2) incomplete antigen repair, fixed tissue for formaldehyde must be fully antigen retrieval to open the antigen epitope, in order to facilitate the combination of antibodies; recommended microwave repair with high fire 4 * 6min try. Someone has done experiments, this is the best time and frequency. If not, but also high-pressure repair;
3) the tissue section itself is low in this antigen;
4) serum blocking time is too long;
5) DAB incubation time is too short;
6) cells are not fully permeable, the antibody failed to fully enter the cell involved in the reaction;
7) begin to do immunohistochemistry, I suggest that you must first be a positive pair of photos, excluding methods other than antibodies.