流感病毒A+B检测试剂盒（免疫层析法）

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流感病毒A+B检测试剂盒（免疫层析法）

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

5，背景强
1）考虑一抗浓度高；
2）然后调整DAB孵育时间；
3）也要考虑血清封闭时间是否过短；
4）适当增加抗体孵育后的浸洗次数和延长浸洗时间等
石蜡制片法是将材料经固定、脱水、透明、浸蜡后包埋在石蜡里面进行切片的方法。此法适用范围，制片手段完备，能将材料切成极薄的片子（可切至2-8微米左右），并能制成连续的片子，这是其它制片法难以达到的，因此在光学显微镜的制片技术上它是zui常用的一种方法。除了一些经不住石蜡切片法中所应用的各种药剂处理的材料不能应用石蜡切片以外，一般的材料都可以用此法制片，但有制作时间较长，操作过程复杂以及材料易发硬或发脆等缺点。该法多采用旋转式切片机进行切片。
石蜡制片操作程序如下：
（一）材料的采集与分割
采集材料应根据制片的目的和要求而定，材料要有代表性，无病虫害或其它损伤，如要观察病理解剖，则要取病斑、病症部位。采下的材料应立即放在标本箱或湿布包起来带回实验室进行分割固定（如有条件也可在试验地采集后进行分割固定）。为使固定液能迅速的渗透材料，材料要进行分割。分割时动作要迅速，以防材料萎蔫。分割块的大小，宜小不宜大，一般不超过1cm3，分割后立即杀死固定。

5, strong background
1) consider the high concentration of primary antibody;
2) Then adjust the DAB incubation time;
3) also consider whether the serum blocking time is too short;
4) appropriate to increase the number of antibody incubation after the dip and extend the immersion time
Paraffin preparation method is the material by the fixed, dehydrated, transparent, immersed wax embedded in paraffin inside the method of slicing. The scope of application of this method, the means of production is complete, the material can be cut into very thin films (can be cut to 2-8 microns), and can be made into a continuous film, which is difficult to achieve by other production methods, so in Light microscopy is technically the most commonly used method of making it. In addition to some paraffin sections can not be used in the treatment of various pharmaceutical agents can not use paraffin sections, the general material can be produced by this method, but with longer production time, the process is complicated and the material is easy to be hard or Crisp and other shortcomings. The law and more rotary slicing machine slicing.
Paraffin preparation procedures are as follows:
(A) material collection and segmentation
Collection materials should be based on the purpose and requirements of the film, the material should be representative, no pests or other damage, such as pathological anatomy to observe, you have to take lesions, the site of the disease. The collected material should be immediay put in the specimen box or wet cloth wrapped up and back to the laboratory for segmentation and fixation (if conditions can also be collected after the trial split and fixed). In order for the fixed liquid to rapidly penetrate the material, the material should be divided. Divide action to quickly, to prevent material wilting. Split the size of the block should be small should not be large, generally not more than 1cm3, killed immediay after the split.