流感B病毒抗原快速检测试剂盒（胶体金法）

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流感B病毒抗原快速检测试剂盒（胶体金法）

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

（四）透明
透明的目的是将脱水剂从材料中除去，使材料透明，增记折光系数；同时便于下步的浸蜡和包埋等程序（因为究竟不能溶解石蜡）。透明剂是一种既能与脱水剂混合，又能与包埋剂混合的药剂。常用的透明剂为二甲苯和绿方，适用原则也是逐级进行，可减少材料收缩。一般步骤是：2/3纯酒精+1/3二甲苯→1/2纯酒精+1/2二甲苯→1/3纯酒精+2/3二甲苯→二甲苯（两次），每级停留时间0.5~2小时或更长些。二甲苯穿透能力强，但又宜使材料收缩变脆，使用时不能在其中停留时间过长。若材料放入二甲苯中时有白色混浊现象发生，说明材料脱水不*，应把材料退回至纯酒精中再行脱水。
（五）浸蜡
浸蜡的目的是逐渐除去透明剂,并为包埋剂所代替。常用的包埋剂是石蜡。浸蜡过程是使石蜡慢慢溶于浸有材料的透明剂中，溶解在透明剂中的石蜡渐渐深入到材料的细胞中去，zui后使透明剂*被石蜡取代，以便切片。
一般所用的石蜡分软蜡（熔点40℃ 以下）和硬蜡（熔点40℃以上）。浸蜡过程一般是从低温到高温、从低熔点到高熔点逐渐进行，这一过程不能操之过急，否则浸蜡不*影响切片。此外，软材料可用熔点较低石蜡，较硬的材料可用熔点较高的石蜡；冬季可用熔点较低的石蜡，夏季可用熔点较高的石蜡。石蜡必须纯净，不含杂质。整个浸蜡过程需在恒温箱内进行（按需要调整温度）。材料zui后一次二甲苯透明后即可浸蜡，浸蜡过程中逐渐使二甲苯挥发，并不断加入纯石蜡，直至石蜡饱和为止。浸蜡时间大约1~2天。

(D) transparent
The purpose of transparency is to remove the dehydrating agent from the material, making the material transparent and adding a refractive index, while facilitating the next step of immersion and embedding (because of the inability to dissolve the paraffin). A clearing agent is an agent that can be mixed with a dehydrating agent and with an embedding agent. Commonly used transparent agent for the xylene and green side, the applicable principle is also step by step, can reduce material shrinkage. The general procedure is: 2/3 pure alcohol +1/3 xylene → 1/2 pure alcohol +1/2 xylene → 1/3 pure alcohol +2/3 xylene → xylene (twice), each stage of stay Time 0.5 ~ 2 hours or longer. Xylene penetration ability, but should make the material shrinkage brittle, when used can not stay in it for too long. If the material into the xylene when white turbidity occurs, indicating that the material is not compley dehydrated, the material should be returned to pure alcohol dehydration again.
(E) Baptist wax
The purpose of the dip wax is to gradually remove the clarifier and replace it with the embedding agent. Common embedding agent is paraffin. Baptist waxing process is slowly dissolve the paraffin in the transparent material soaked in the paraffin dissolved in the transparent agent gradually into the material cells, and finally to make the transparent agent compley replaced by paraffin, in order to slice.
Paraffin wax generally used points (soft point below 40 ℃) and hard wax (melting point of 40 ℃ or more). Baptist waxing process is generally from low temperature to high temperature, from low melting point to high melting point gradually, this process can not be hasty, or not compley affect the slicing dip wax. In addition, the lower melting point of soft materials available paraffin, harder materials available higher melting paraffin; available winter melting lower paraffin, paraffin melting point in summer available higher. Paraffin wax must be pure, free of impurities. The whole process of waxing needs to be carried out in the incubator (adjust the temperature as needed). After the last time the material is transparent, xylene can be dipped in wax, the xylene is gradually volatilized during the dipped wax, and pure paraffin is continuously added until the wax is saturated. Baptist wax time of about 1 to 2 days.