甲乙型流感病毒诊断试剂盒（爱思普林）

广州健仑生物科技有限公司

广州健仑长期供应各种流感检测试剂，包括进口和国产的品牌，主要包括日本富士瑞必欧、日本生研、美国BD、美国NovaBios、美国binaxNOW、日本积水、日本荣研、美国OSOM、芬兰、爱思普林、英国Clearview、凯必利、广州创仑等主流品牌。

甲乙型流感病毒诊断试剂盒（爱思普林）

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

1、 一抗从4度拿出后，为什么要进行37度复温？
（1）一方面，防止切片从4度直接放入PBS易脱片；
（2）另一方面，使抗原抗体结合更稳定。一般不需要，但对表达较弱的抗原可能有用，4度和37度时分子运动方式不同，前者分子碰撞机率和运动速度小于后者，后者结合更快，但敏感性也提高了并易造成非特异染色。
（3）其实，我更赞同后一种说法，因为我尝试把肝脏或睾丸片子从4度过夜拿出后，直接用PBS洗没发生过脱片现象。
2、切片染色后背景太深，如何区分特异性与非特异性着色？
全片着色是指整个切片全都染上了颜色，着色的强度可深可浅，总之，分不清那些组织是阳性那些组织是阴性。出现这种现象的原因有：
（1）抗体浓度过高：一抗浓度过高是常见的原因之一。解决办法是，每次使用新抗体前应当对其工作浓度进行测试，使每一抗体个体化，找到适合自己实验室的理想工作浓度，既使是即用型的抗体也应如此，不能只简单的按说明书进行染色。
（2）抗体孵育时间过长或温度较高：解决办法是，严格执行操作规程，随身佩带报时表或报时钟，及时提醒，避免因遗忘而造成时间延长。现在流行的二步法（Polymer）敏感性很高，要求一抗孵育的时间不是传统的1小时，而是30分钟，因此，要根据染色结果进行调整。

1, the first antibody out from 4 degrees, why 37 degrees rewarming?
(1) On the one hand, to prevent slicing directly into PBS from 4 degrees easy to strip;
(2) On the other hand, antigen-antibody binding is more stable. Generally not needed, but may be useful for poorly expressed antigens, 4 and 37 degrees when the molecular movement in different ways, the former molecular collision probability and speed less than the latter, the latter combination of faster, but the sensitivity also increased and easy Cause non-specific staining.
(3) In fact, I agree with the latter statement, because I tried to wash the liver or testis out of the night after 4 degrees, directly washed with PBS did not have the phenomenon of delamination.
2, slice background is too dark, how to distinguish between specific and non-specific coloring?
Whole-piece coloring refers to the entire section of the color dyed, the depth of the shading can be shallow, in short, can not l those organizations are positive those organizations are negative. The reasons for this phenomenon are:
(1) antibody concentration is too high: a high concentration of anti-one is a common cause. The solution is to test the working concentration before each use of the new antibody so that each antibody is individualized to find the ideal working concentration for your laboratory, even for ready-to-use antibodies, not just simple According to the instructions for staining.
(2) antibody incubation time is too long or high temperature: The solution is to strictly enforce the rules, it is best to wear timepieces or clock, timely reminder to avoid the delay caused by the forgotten. Now the popular two-step method (Polymer) high sensitivity, requiring a first antibody incubation time is not the traditional one hour, but 30 minutes, therefore, according to the staining results to be adjusted.