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食物感染流感病毒诊断试剂盒（日本荣研）

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5、DAB显色后发现切片着色不均匀：如一片着色，一片不着色或染色浅？
着色不均匀可能与你的实验手法有很大关系，可能原因有：
（1）切出的片子厚度本身可能就不一样，切出一片厚，一片薄，这样可能造成着色深浅不一。
（2）有可能是你 显色液底物加到片子上后没有摇匀，造成局部底物浓度不*，所以加完显色液后将片子来回左右晃动几下，使片子上所有区域的底物浓度*。
（3）如果你的片子是中间的染色深，靠近边上的浅，那有可能是你蜡圈画的太小，显色液覆盖的面积不过大而产生了边缘效应。zui外侧的组织片离显色液外缘0.5 cm。
良好的免疫组化染色切片是正确判断染色结果的基础和前提。由于免疫组化染色过程中存在很多步骤或环节，每一个步骤或环节都可能影响到染色的zui终结果，因此，要做好一张高质量的免疫组化切片并不是一件非常容易的事。需要病理技术员和病理医生密切配合、相互协调、共同努力才能保证做出合格的免疫组化切片。虽然免疫组化染色可以存在各种各样的问题，但从染色的结果看，一般可分为两类：无色片（即无阳性信号）和“ 杂音”染色片（有阳性信号）。

5, DAB color chips and found uneven coloring: If a piece of coloring, a non-coloring or staining?
Uneven coloring may be related to your experimental approach, probably for a number of reasons:
(1) the thickness of the cut out film itself may not be the same, cut out a thick, thin, which may cause shades of color.
(2) it may be your chromogenic substrate added to the film after shaking did not shake, resulting in inconsistent local substrate concentration, so after the end of the color liquid is best to move back and forth around the film a few times so that all areas on the film The same substrate concentration.
(3) If your film is deep in the middle, near the edge of the shallow, it may be your wax ring painting is too small, the color of the liquid coverage area is too large and have an edge effect. The outermost tissue piece is best 0.5 cm from the outer edge of the color reagent.
Good immunohistochemical staining is the correct basis for judging the results of staining and the premise. Due to the many steps or steps involved in immunohistochemical staining, each step or step may affect the final result of staining. Therefore, it is not easy to make a high-quality immunohistochemical slice. Requires pathological technicians and pathologists in close cooperation, coordination and work together to ensure that qualified immunohistochemical slices. Although there are a variety of problems with immunohistochemical staining, there are generally two types of staining that result from staining: colorless (ie, no positive) and "murmur" (positive).