PCR试剂 流感甲乙型/合胞病毒AB型四重检测试剂盒

广州健仑生物科技有限公司

Ready to use lyo master mix (8-well strips each) for detection of influenza A virus, influenza B virus and human respiratory syncytial viruses A and B including internal control.
准备使用lyo master混合物（每个8孔条）检测甲型流感病毒，乙型流感病毒和人类呼吸道合胞病毒A和B（包括内部对照）。
Principle
Multiplex real-time PCR for detection of pathogen genes by TaqMan® technology
Targets
Lyo mastermix:
influenza A virus
influenza B virus
human respiratory syncytial virus A/B
internal Control
Specimen
This test is for use with extracted nucleic acid from respiratory samples (throat/nasal swabs, bronchoalveolar lavage and sputum) of human origin
Storage
Liquid and lyophilised components: 2-8°C until expiration date
Sealing foils: room temperature
Shelf life
12 months from manufacture

PCR试剂 流感甲乙型/合胞病毒AB型四重检测试剂盒

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PCR试剂

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

1．  固定：用4%的多聚甲醛固定液。对于冰冻切片，甲醛固定有时比冰冻丙酮好；但对于不同的组织和抗原，可选用不同的固定液。
Bouin S固定液：饱和苦味酸750ml，甲醛250ml，冰醋酸50ml，其对组织的穿透力较强，固定较好，结构完整，但因偏酸，对抗原有一定损害，且组织收缩明显，不适于组织标本的长期保存。 
PLP液：即高碘酸钠-赖氨酸-多聚甲醛，适于固定石蜡切片。适于富含糖类组织，对超微结构及许多抗原的抗原性保存较好。 
2．组织脱水，透明：时间不能太长，否则在切片时容易碎片，切不完整。 
3．切片时展片：有些组织在切片后难以在水中展开，这时可适当地在水中加入几滴乙醇。 
4．烤片：60℃ 30分钟或37℃ 过夜，温度太高或时间太长，抗原容易丢失。 
5．蜡块及切片的保存：在4℃保存 
6．脱片问题： Poly-L-Lysine(多聚赖氨酸)为目前免疫组化染色工作中zui常用的一种防脱片剂，6ml的多聚赖氨酸溶液可按1:10稀释成60ml的工作液，适合于需要酶消化、微波、高温高压的防脱片处理。如不行，可用双重处理（APES和Poly-L-Lysine）的切片。在以上两种条件都行不通的情况下，可用如下方法：切片在脱蜡前，放在APES 1：50 丙酮溶液中浸泡3分钟，晾干，即可进行下一步。 
7．灭活内源性酶：HRP系统：3%双氧水灭活；AP系统：3%HAc灭活。 
8．暴露抗原：对于石蜡切片的免疫组化实验时，必须采用高温加热抗原修复，这将有助于暴露抗原决定簇，从而增加免疫组化染色的强度(不同抗体的*修复液请参阅抗体说明书)。对于不同的组织，不同的抗原，不同的抗体，所采用的方法应不一样，可进行热修复、胰酶消化、既不修复也不消化。胶原还可以用胃蛋白酶消化等。 

1. Fixed: It is best to use 4% paraformaldehyde fixative. For frozen sections, formaldehyde fixation is sometimes better than frozen acetone; however, different fixatives may be used for different tissues and antigens.
Bouin S fixed solution: saturated picric acid 750ml, formaldehyde 250ml, glacial acetic acid 50ml, the penetration of its stronger, better fixed, structural integrity, but due to acid, have some damage to the antigen, and tissue shrink significantly, Not suitable for long-term preservation of tissue specimens.
PLP solution: sodium periodate - lysine - paraformaldehyde, suitable for fixed paraffin sections. Suitable for rich in carbohydrate tissue, the antigenicity of the ultrastructure and many antigens is better preserved.
2. Tissue dehydration, transparent: the time can not be too long, otherwise easy to fragment in the slice, cut incomplete.
3. Splinting: Some tissues are difficult to unfold in water after slicing, when a few drops of ethanol are properly added to the water.
4. Baked pieces: 60 ℃ 30 minutes or 37 ℃ overnight, the temperature is too high or too long, the antigen is easy to lose.
5. Preservation of wax blocks and slices: best preserved at 4 ℃
6. The problem of delamination: Poly-L-Lysine (Polylysine) is the most commonly used anti-drop tablet for immunohistochemical staining. 6ml of polylysine solution can be diluted 1:10 into 60ml The working fluid, suitable for digestion, microwave, high temperature and pressure of the anti-off film processing. If not, double-stained sections (APES and Poly-L-Lysine) can be used. In the case of the above two conditions are not feasible, the following method can be used: slice before dewaxing, put in APES 1:50 acetone solution soak for 3 minutes, dry, you can proceed to the next step.
7. Inactivation of endogenous enzymes: HRP system: 3% hydrogen peroxide inactivated; AP system: 3% HAc inactivated.
8. Exposure to Antigens: For immunohistochemistry of paraffin sections, high-temperature heating of the antigen must be used to repair the antigen, which will help to expose the antigenic determinants and thereby increase the intensity of immunohistochemical staining (see Antibody manual for optimal antibody repair of different antibodies ). For different tissues, different antigens, different antibodies, the method used should be different, can be heat repair, trypsin digestion, neither repair nor digestion. Collagen can also be digested with pepsin and so on.