PCR试剂 肠道病毒/帕氏病毒/腺病毒核酸检测试剂盒

广州健仑生物科技有限公司

准备使用lyo master混合物（每个8孔条）检测肠道病毒，帕氏病毒和腺病毒包括内部对照。
Ready to use lyo master mix (8-well strips each) for detection of enterovirus, parechovirus and adenovirus including internal control.
Principle
Multiplex real-time PCR for detection of pathogen genes by TaqMan® technology
Targets
Lyo mastermix:
enterovirus
human parechovirus
human adenovirus
internal control
Specimen
This test is for use with extracted nucleic acid from CSF, blood (with heparin), throat swabs, sputum and stools of human origin
Storage
Liquid components: -20°C
Lyophilised components: 2-8°C
Sealing foils: room temperature
Shelf life
12 months from manufacture

PCR试剂 肠道病毒/帕氏病毒/腺病毒核酸检测试剂盒

我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。

欢迎咨询

欢迎咨询2042552662

PCR试剂

想了解更多的产品及服务请扫描下方二维码：

【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

【】 
【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

  （1）高压热修复 在沸水中加入EDTA（pH8.0）或0.01M枸橼酸钠缓冲溶液（pH6.0）。盖上不锈钢高压锅的盖子，但不进行锁定。将玻片置于金属染色架上，缓慢加压，使玻片在缓冲液中浸泡5分钟，然后将盖子锁定，小阀门将会升起来。10分钟后，去除热源，置入凉水中，当小阀门沉下去后打开盖子。本方法适用于较难检测或核抗原的抗原修复。
   （2）煮沸热修复 电炉或者水浴锅加热0.01M枸橼酸钠缓冲溶液（pH6.0）至95℃左右，放入组织芯片加热10~15分钟。
   （3）微波热修复 在微波炉里加热0.01M枸橼酸钠缓冲溶液（pH6.0）至沸腾后将组织芯片放入，断电，间隔5~10分钟，反复1-2次。适用的抗原有：AR，Bax，Bcl-2，C-fos，X-jun，C-kit，C-myc，E-cadherin，Chromogranin A，Cyclin，ER，Heat shock protein，HPV，Ki-67，MDMZ，p53，p34，p16，p15，P-glycoprotein，PKC，PR，PCNA，ras，Rb，TopoismeraseⅡ等。是以高温，高压对常规固定的石蜡切片进行抗原修复或复原的一种非蛋白酶消化以提高抗原抗体阳性检测的一种方法和技术手段。甲醛和蛋白水解交联过程中氨基酸侧链上的某些基团(抗原决定簇)如咪唑、吲哚等基团受到影响，通过120℃高温或强碱处理后，可使交联打开。
   2）酶消化方法 常用0.1%胰蛋白酶和0.4%胃蛋白酶液。胰蛋白酶使用前预热至37℃，切片也预热至37℃，消化时间约为5~30分钟；胃蛋白酶消化37℃时间为30分钟。适用于被固定遮避的抗原，其中有：Collagen，Complement，Cytokeratin，C-erB-2，GFAP，LCA，LN等。

(1) High-pressure heat repair Add EDTA (pH8.0) or 0.01M sodium citrate buffer solution (pH6.0) to boiling water. Cover the stainless steel autoclave lid without locking. Place the slide in a metal-stained rack, apply pressure slowly and allow the slide to soak for 5 minutes in the buffer, then lock the lid and the small valve will lift. After 10 minutes, remove the heat and place in cold water and open the lid when the small valve is lowered. The method is more difficult to detect antigen or antigens antigen.
   (2) boil hot repair electric furnace or water bath heating 0.01M sodium citrate buffer solution (pH6.0) to about 95 ℃, into the tissue chip heating 10 to 15 minutes.
   (3) Microwave Heat Repair In the microwave oven heating 0.01M sodium citrate buffer solution (pH6.0) to the chip after boiling into the tissue, power, interval of 5 to 10 minutes, repeated 1-2 times. Antigens such as AR, Bax, Bcl-2, C-fos, X-jun, C-kit, C-myc, E-cadherin, Chromogranin A, Cyclin, ER, Heat shock protein, MDMZ, p53, p34, p16, p15, P-glycoprotein, PKC, PR, PCNA, ras, Rb and Topoismerase II. Is a high temperature, high pressure on the routine fixed paraffin sections antigen retrieval or recovery of a non-protease digestion to improve antigen-antibody positive detection of a method and technical means. Formaldehyde and proteolytic crosslinking some of the amino acid side chains (epitopes) such as imidazole, indole and other groups are affected, through 120 ℃ high temperature or alkali treatment, you can make the cross-link open.
   2) enzyme digestion method commonly used 0.1% trypsin and 0.4% pepsin solution. Trypsin was preheated to 37 ° C before use and the pellet was also preheated to 37 ° C with a digestion time of about 5 to 30 minutes and pepsin digestion at 37 ° C for 30 minutes. It is suitable for antigens that are fixedly sheltered and include: Collagen, Complement, Cytokeratin, C-erB-2, GFAP, LCA, LN and others.