PCR试剂 六联病毒性胃肠炎PCR核酸检测试剂盒

广州健仑生物科技有限公司

双管多重加附加单抗检测诺如病毒G1，G2，星状病毒，轮状病毒，腺病毒，沙波病毒和内部对照。
Two tube multiplex plus add-on singleplex for detection of norovirus G1, G2, astrovirus, rotavirus, adenovirus, sapovirus and internal control.

PCR试剂 六联病毒性胃肠炎PCR核酸检测试剂盒

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PCR试剂

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

一、非特异性染色的主要因素 
组织的非特异性染色的机理很复杂，其产生的原因主要可分为以下几点： 
（1）一部分荧光素未与蛋白质结合，形成了聚合物和衍化物，而不能被透析除去。 
（2）抗体以外的血清蛋白与荧光素结合形成荧光素脲蛋白，可与组织成分结合。 
（3）除去检查的抗原以外，组织中还可能存在类属抗原（如Forssman氏抗原），可与组织中特异性抗原以外之之相应抗体结合。 
（4）从组织中难于提纯抗原性物质，所以制备的免疫血清中往往混杂一些抗其他组织成分的抗体，以致容易混淆。 
（5）抗体分子上标记的荧光素分子太多，这种过量标记的抗体分子带过多的阴离子，可吸附于正常组织上而呈现非特异性染色。 
（6）荧光素不纯，标本固定不当等。 

First, non-specific staining of the main factors
Tissue non-specific staining mechanism is very complex, its causes can be divided into the following points:
(1) A portion of the fluorescein is not bound to the protein, forming a polymer and derivative that can not be removed by dialysis.
(2) Serum proteins other than antibodies bind to fluorescein to form fluorescein urea, which binds to tissue components.
(3) In addition to the antigen to be examined, there may also be a generic antigen (such as Forssman's antigen) in the tissue that binds to a corresponding antibody other than the specific antigen in the tissue.
(4) It is difficult to purify the antigenic substance from the tissue, so the prepared immune serum is often mixed with antibodies against other tissue components, so that it is easy to be confused.
(5) There are too many fluorescein molecules labeled on the antibody molecule. This excess labeled antibody molecule with too many anions can be adsorbed on normal tissues and present nonspecific staining.
(6) impure fluorescein, specimen fixed improperly.