核酸试剂 艰难梭菌多重检测试剂盒（PCR-荧光探针法）

广州健仑生物科技有限公司

单管多重检测艰难梭菌及内部对照。
One tube multiplex for detection of Clostridium difficile and internal control.

核酸试剂 艰难梭菌多重检测试剂盒（PCR-荧光探针法）

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

解决阴性染色的问题非常简单，就是设立“阳性对照”。如果阳性对照有了表达，说明染色的全过程和所有试剂都没有问题。如果此时测试片仍为阴性，便是真实的阴性，说明组织或细胞没有相应的抗原表达。反之，如果阳性对照没有着色，表明染色过程中某个或某些步骤出了问题或试剂出了问题。应一一寻找原因。阳性对照包括两种，一种称为“自身对照”或“内部对照”，这是指在测试的切片中本身就存在已知的抗原，如正常淋巴结中存在T和B细胞抗原，CD20或CD3都应该有表达。自身对照是一种比较理想的对照，对照和测试组织或细胞都在同一张切片中，都处于相同的试验条件下，结果更可靠也更具有可比性。在选择自身对照片时选择既有病变组织同时又有正常组织的部分，这样有利于对比。另一种称为“外部对照”，有时在测试的切片中不存在已知的抗原，如在胃的标本中怀疑是恶性黑色素瘤，需要用HMB45或Mart-1来检测，在正常的胃组织中本身不存在相关的抗原，如果病变出现阳性反应结果，尚能提示是恶黑，但是如果出现阴性结果，就无法确定是本身组织中不含黑色素瘤抗原，还是技术问题。因此，应另外设立一个已知的阳性对照。这种在测试组织之外的阳性对照称为“外部对照”。在实际工作中需要设立外部对照的情况很多，如果每一种抗体都要选不同的阳性对照，工作量会很大。为了解决这个问题，目前国内外有单位将多种不同组织集成在一起，制成多组织切片、“腊肠”“春卷”切片、组织芯片等，其连续切片储备待用，需要时取出一张便可作为阳性对照。另外，比较简单的方法，是采用阑尾作为阳性对照，因为与人体其它组织器官比较阑尾包含的组织种类较多，如有上皮、淋巴组织、平滑肌、间质、神经、间皮、血管等。一张阑尾切片可以检测大多数常用的抗体。

To solve the problem of negative staining is very simple, is to set up a "positive control." If the positive control is expressed, indicating that the whole process of dyeing and all reagents are no problem. If the test piece is still negative at this time, it is a true negative, indicating that the tissue or cell does not have the corresponding antigen expression. Conversely, if the positive control is not colored, it indicates that there was a problem or some problem with the reagents during the dyeing process. One by one should find the reason. Positive controls include two, one called "self-control" or "internal control", which means that there are known antigens themselves in the test section, such as the presence of T and B cell antigens in normal lymph nodes, CD20 or CD3 All should be expressed. Self-control is an ideal control, the control and test tissues or cells are in the same slice, are in the same experimental conditions, the result is more reliable and more comparable. In the choice of their own photos of the best choice both the lesion and normal tissue part, which is conducive to the comparison. Another known as the "external control", and sometimes there is no known antigen in the test section, as in gastric specimens suspected of malignant melanoma, the need to use HMB45 or Mart-1 to detect, in normal gastric tissue Itself does not exist in the relevant antigen, if the lesions appear positive results, still can suggest evil, but if there is a negative result, it is not sure whether the tissue itself contains no melanoma antigen, or technical problems. Therefore, another known positive control should be set up. This positive control outside the test tissue is called the "external control." There are many situations where external controls need to be set up in practice. If each positive antibody is tested for a different positive control, the workload will be heavy. In order to solve this problem, at present, some domestic and foreign units integrate many different kinds of organizations to make multi-tissue slices, "sausage", "spring roll" slices, tissue chips and the like, and their serial slices are reserved for use. Can be used as a positive control. In addition, the relatively simple method is to use the appendix as a positive control, because compared with other tissues and organs of the human body appendix contains more tissue types, such as epithelial, lymphoid tissue, smooth muscle, interstitial, nerve, blood vessels, mesothelium and so on. An appendectomy can detect most commonly used antibodies.