风麻疹病毒二联检诊断试纸（酶联免疫法）

广州健仑生物科技有限公司

广州健仑长期供应各种ELISA试剂盒，主要代理进口和国产品牌的流行病毒ELISA检测试剂盒。例如：甲乙型流感病毒酶联免疫法检测试剂盒、黄热病毒酶联免疫法检测试剂盒、诺如病毒酶联免疫法检测试剂盒、登革病毒酶联免疫法检测试剂盒、基孔肯雅病毒酶联免疫法检测试剂盒、结核杆菌酶联免疫法病毒检测试剂盒、孢疹病酶联免疫法检测试剂盒、西尼罗河病毒酶联免疫法检测试剂盒、呼吸道合胞病毒酶联免疫法检测试剂盒、冠状病毒酶联免疫法检测试剂盒等等。虫媒体染病系列、呼吸道病原体系列、发热伴出疹系列、消化道及食源感染系列。

检验原理风麻疹病毒二联检诊断试纸（酶联免疫法）

用抗原包被微量板孔，制成固相载体。加患者血清到板孔中，其所含的抗体特异性地与固相载体中现存抗原结合，形成免疫复合物。除去多余物质后，加入结合了碱性磷酸酶的IgG、IgA或IgM抗体，使之与上述免疫复合物反应。洗板，除去多余的结合物，加入底物（对硝基苯磷酸盐）。其与酶结合的免疫复合物反应，产生有颜色产物，颜色强度与特异性抗体含量成正比。

产品规格：96T/盒

存储条件：4-8℃

ELISA法：用灭活风疹病毒抗原包被的载体可与被检样品中的特异性抗体结合，用酶标记的抗人免疫球蛋白与底物检测相应抗体。
试剂
除灭活风疹病毒抗原包被的微孔板，阴、阳性参考血清外，其余同一般ELISA。
操作方法
（1）样品采集，作过筛试验仅需采集单份血样，但若需判断病毒感染者的免疫状态，需对可疑风疹患者在出现斑疹后3天内以及随后的14～21天分别取样同时检测。
（2）同一般ELISA，在对照和样品各孔中加PBS 50μl，继加样品10μl，25℃保温45min，洗涤、吸干。
（3）各孔加酶标记物250μl，同法保温和洗涤。
（4）再加pNPP底物溶液250μl，同法保温和洗涤后，加1mol/L氢氧化钠50μl中止反应，测定各孔吸光度值405nm，判断被测样品结果。
（5）若为阳性结果，可将样品作进一步稀释后作抗体滴度测定，比较先后两份样品结果，并进行判断（表1）：

判定
1.风疹病毒的IgG和lgM抗体均为阳性，或者是IgG抗体滴度≥1：512，表明有风疹病毒近期感染。
2.风疹病毒的IgG和IgM抗体均为阴性，表明没有受过风疹病毒感染。
3.风疹病毒的lgG抗体滴度<1：512，lgM抗体为阴性，说明有过既往感染史。
4.此外，再次感染风疹病毒，由于只出现短暂的IgM抗体或出现的水平很低，所以不易查出。因此风疹病毒IgG抗体滴度在双份血清中有4倍以上升高，那么无论lgM抗体是否为阳性，都是风疹病毒近期感染的指标。

ELISA method: The carrier coated with inactivated rubella virus antigen can be combined with the specific antibody in the sample to be tested, and the corresponding antibody is detected with enzyme-labeled anti-human immunoglobulin and substrate.
Reagents
In addition to the inactivated rubella virus antigen coated microplate, negative and positive reference serum, the rest of the same ELISA.
Method of operation
(1) sample collection, sieve test only need to collect a single blood sample, but if you need to determine the immune status of the virus-infected persons, the need for suspected rubella patients within 3 days after the emergence of rash and subsequent sampling of 14 to 21 days at the same time Detection.
(2) In the same ELISA, add PBS 50μl to each well of control and sample, add 10μl of sample, incubate at 25 ℃ for 45min, wash and dry.
(3) Each well plus enzyme labeling 250μl, with the law insulation and washing.
(4) add pNPP substrate solution 250μl, the same method of incubation and washing, add 1mol / L sodium hydroxide 50μl reaction was stopped, the determination of the absorbance of each well 405nm value, determine the test sample results.
(5) If the result is positive, the sample can be further diluted for antibody titers. The results of the two samples are compared and judged (Table 1):

determination
1. Rubella IgG and lgM antibodies are positive, or IgG antibody titers ≥ 1: 512, indicating a recent infection with rubella virus.
Rubella virus IgG and IgM antibodies were negative, indicating that they have not been rubella virus infection.
3. Rubella virus lgG antibody titer <1: 512, lgM antibody negative, indicating that there was a history of previous infections.
4. In addition, re-infection with rubella virus, due to the emergence of transient IgM antibodies or the emergence of low levels, so difficult to detect. Therefore, rubella virus IgG antibody titers in double serum increased more than four times, then whether lgM antibodies are positive, are indicators of recent infection with rubella virus.

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103

病毒存在于出疹前5～7天病儿唾液及血液中，但出疹2天后就不易找到。风疹病毒在体外生活力很弱，但传染性与麻疹一样强。好发于5岁以下的婴幼儿，6个月以内婴儿因有来自母体的抗体获得抵抗力，很少发病。一次得病，可终身免疫，很少再次患病。春夏之交，风疹病毒也在欲动，它会伴随人的咳嗽和喷嚏而漂浮在空气中。抵抗力较弱的人吸入风疹病毒后，经过2~3周的潜伏期，便开始出现症状。先是全身不适，继而出现发热、耳后及枕部淋巴结肿大，并有淡红色细点状丘疹，短期内扩展到全身，奇痒难耐或微痒，多在2~3天内消退，*迹。由于风疹的症状和体征与感冒及荨麻疹相似，因而不太引起人们的重视。
风疹从接触感染到症状出现，要经过14～21天。病初1～2天症状很轻，可有低热或中度发热，轻微咳嗽、乏力、胃口不好、咽痛和眼发红等轻度上呼吸道症状。病人口腔粘膜光滑，无充血及粘膜斑，耳后、枕部淋巴结肿大，伴轻度压痛。通常于发热1～2天后出现皮疹，皮疹先从面颈部开始，在24小时蔓延到全身。皮疹初为稀疏的红色斑丘疹，以后面部及四肢皮疹可以融合，类似麻疹。出疹第二天开始，面部及四肢皮疹可变成针尖样红点，如猩红热样皮疹。
皮疹一般在3天内迅速消退，留下较浅色素沉着。在出疹期体温不再上升，病儿常无疾病感觉，饮食嬉戏如常。风疹与麻疹不同，风疹全身症状轻，无麻疹粘膜斑，伴有耳后、颈部淋巴结肿大。
风疹是病毒性传染病，临床特点是：全身症状轻微，皮肤红色斑丘疹及枕后、耳后、颈后淋巴结肿大伴触痛，合并症少见。病毒可通过胎盘传给胎儿而致各种先天缺陷，称为先天性风疹综合征。

The virus is present in the saliva and blood of sick children 5 to 7 days before the rash but is not easy to find after 2 days of rash. Rubella virus has a weak viability in vitro but is as contagious as measles. Occur in infants and children under 5 years of age, within 6 months of infants derived from the mother's antibody resistance, rarely disease. Once sick, lifelong immunity, rarely again sick. At the turn of spring and summer, the rubella virus is just around the corner, floating in the air with coughing and sneezing. People with weaker immunity inhaled rubella virus, after 2 to 3 weeks of incubation period, began to show symptoms. First, general malaise, followed by fever, ear and occipital lymph nodes, and fine pink papules, extended to the body within a short period of time, itchy emboldened or slightly itchy, mostly in 2 to 3 days subsided, leaving no trace. Because the symptoms and signs of rubella are similar to those of flu and urticaria, they are less of a cause for concern.
Rubella from infection to symptoms, to go through 14 to 21 days. 1 to 2 days early disease symptoms are very mild, may have low or moderate fever, mild cough, fatigue, poor appetite, sore throat and eye redness mild upper respiratory symptoms. Patients with smooth oral mucosa, no congestion and mucosal plaque, ear, occipital lymph nodes, with mild tenderness. Usually 1 to 2 days after the fever rash, rash first from the face and neck, spread to the body in 24 hours. Rash at early sparse red rash, after facial and limb rash can be integrated, similar to measles. Rash the next day, the face and limb rash can become a needle-like red dot, such as scarlet fever-like rash.
The rash subsided rapidly within 3 days, leaving a more pale pigmentation. In the rash of the body temperature is no longer rising, sick children often have no sense of disease, diet playful as usual. Rubella and measles are different, rubella systemic symptoms of light, no measles mucosa, accompanied by ear, neck lymph nodes.
Rubella is a viral infectious disease, clinical features are: minor symptoms, red rash and occiput skin, behind the ear, neck and neck lymph nodes with tenderness, complications rare. The virus can be passed to the fetus through the placenta caused by a variety of birth defects, known as congenital rubella syndrome.