细菌性脑膜炎3联荧光PCR检测试剂盒

广州健仑生物科技有限公司

单管多重检测和定量肺炎链球菌，脑膜炎奈瑟氏菌，流感嗜血杆菌和内部控制。
One tube multiplex for detection AND quantification of Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influenzae and internal control.

细菌性脑膜炎3联荧光PCR检测试剂盒

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

3．滤纸保藏法
（1）将滤纸剪成0．5×1．2cm的小条，装入0．6×8cm的安瓿管中，每管1—2张，塞以棉塞，1．05kg/cm2>，121．3℃灭菌30分钟。
（2）将需要保存的菌种，在适宜的斜面培养基上培养，使充分生长。
（3）取灭菌脱脂牛乳1—2ml滴加在灭菌培养皿或试管内，取数环菌苔在牛乳内混匀，制成浓悬液。
（4）用灭菌镊子自安瓿管取滤纸条浸入菌悬液内，使其吸饱，再放回至安瓿管中，塞上棉塞。
（5）将安瓿管放入内有五氧化二磷作吸水剂的干燥器中，用真空泵抽气至干。
（6）将棉花塞入管内，用火焰按图Ⅶ-13熔封，保存于低温下。
（7）需要使用菌种，复活培养时，可将安瓿管口在火焰上烧热，滴一滴冷水在烧热的部位，使玻璃破裂，再用镊子敲掉口端的玻璃，待安瓿管开启后，取出滤纸，放入液体培养基内，置温箱中培养。

This method is practical and effective. Molds, actinomycetes, spores and bacteria can be preserved more than 2 years immortality, yeast can be stored for 1-2 years, the general non-spore bacteria can be stored for about 1 year, even with the usual method is difficult to preserve meningococci, at 37 ℃ Thermostat, can also be stored for 3 months. This method has the advantage of making a simple, no special equipment, and do not need to move frequently. The disadvantage is that the preservation must be placed upright, occupy a larger location, but also inconvenient to carry. Care should be taken when the culture rings are burned in the flame after the paraffin is removed from the liquid paraffin and the culture is easily splashed together with residual liquid paraffin.
3. Filter paper preservation method
(1) The filter paper cut into 0.5 × 1.2cm strip into the 0.6 × 8cm ampoule tube, each tube 1-2, tampon plug, 1.05kg / cm2>, 121 Sterilize at 3 ° C for 30 minutes.
(2) The need to save the strains, cultured in the appropriate slant medium, so that full growth.
(3) Take 1-2ml sterile skim milk drop in sterile petri dishes or test tube, take a few ring bacteria in the milk mix, made into a suspension.
(4) using sterile tweezers to take the filter paper from ampoule tube immersed in the bacterial suspension, make it saturated, and then back into the ampoule tube, tampon plug.
(5) Place the ampoule into a desiccator containing phosphorus pentoxide as a water-absorbing agent and pump it to dryness with a vacuum pump.
(6) The cotton into the tube, flame with Figure VII-13 seal, stored at low temperature.
(7) need to use bacteria, the resurrection culture, the ampoule nozzle can be heated in the flame, a drop of cold water in the hot parts, so that the glass rupture, and then tweezers knock the mouth of the glass until the ampoule tube open , Remove the filter paper, into the liquid medium, incubator incubator.
Bacteria, yeasts, filamentous fungi can be preserved by this method, the first two can be stored for about 2 years, some filamentous fungi can even be preserved for 14-17 years. This method is more liquid nitrogen, freeze-drying method is simple, does not require special equipment.