克里米亚刚果出血热病毒荧光PCR检测试剂盒

广州健仑生物科技有限公司

One tube multiplex for detection of Crimean Congo haemorrhagic fever virus and internal control.

单管多重检测克里米亚刚果出血热病毒和内部控制。

克里米亚刚果出血热病毒荧光PCR检测试剂盒

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7、颜色改变单位法（colour change unit,简称CCU）
这种方法通常用于很小，用一般的比浊法无法计数的微生物，比如支原体等，因为支原体的液体培养物是*透明的，呈现为清亮透明红色，因此无法用比浊法来计数，由于支原体固体培养很困难，用cfu法也不容易计数，因此需要用特殊的计数方法，即CCU法。它是以微生物在培养基中的代谢活力为指标，来计数微生物的相对含量的，下面以解脲脲原体为例，简单介绍其操作：
（1）.取12只无菌试管，每一管装1.8ml解脲脲原体培养基。
（2）.在*管加入0.2ml待测解脲脲原体菌液，充分混匀，从中吸取0.2ml加入第二管，依次类推，10倍梯度稀释，一直到zui末一管
（3）.于37度培养，以培养基颜色改变的zui末一管作为待测菌液的CCU，也就是支原体的zui大代谢活力，比如第六管出现颜色改变，他的相对浓度就是10的6次方CCU/ml.
一般来说，比浊法和菌落计数法就可以满足绝大多数细菌的计数，但是对支原体这样比较特殊的微生物，用CCU法比较合适。
1.1.常量肉汤稀释法
1.1.1.抗菌药物贮存液制备 抗菌药物贮存液浓度不应低于1000μg/ml(如1280μg/ml)或10倍于zui高测定浓度。溶解度低的抗菌药物可稍低于上述浓度?？咕┪镏苯庸鹤猿袒蛳喙鼗埂Ｋ杩咕┪锶芤毫炕蚍奂亮靠晒浇屑扑?。例如：需配制100 ml浓度为1280μg/ml的抗生素贮存液，所用抗生素为粉剂，其药物的有效力为750μg/mg。用分析天平精确称取抗生素粉剂的量为182.6 mg。根据公式计算所需稀释剂用量为：（182.6 mg×750μg/ml）/1280μg/ml=107.0ml，然后将182.6 mg抗生素粉剂溶解于107.0ml稀释剂中。制备抗菌药物贮存液所用的溶剂和稀释剂见表5。配制好的抗菌药物贮存液应贮存于-60℃以下环境，保存期不超过6个月。
1.1.2.药敏试验用抗菌药物浓度范围 根据NCCLS抗菌药物敏感性试验操作标准，药物浓度范围应包含耐药、中介和敏感分界点值，特殊情况例外。

7, color change unit method (color change unit, referred to as CCU)
This method is generally applied to microorganisms such as mycoplasma which can not be counted by the ordinary turbidimetry because liquid cultures of mycoplasma are compley transparent and clear and bright red and thus can not be counted by turbidimetry. Because mycoplasma solid culture is very difficult to use cfu method is not easy to count, so the need for a special counting method, that CCU method. It is the microbial metabolic activity in the medium as an indicator to count the relative content of microorganisms, the following Ureaplasma urealyticum, for example, a brief description of its operation:
(1) Take 12 sterile tubes, each tube 1.8ml Ureaplasma urealyticum medium.
(2) 0.2 ml of the Ureaplasma urealyticum solution to be tested is added into the first tube, thoroughly mixed, and 0.2 ml of the Ureaplasma urealyticum is taken into the second tube, followed by a 10-fold gradient dilution until the last tube
(3) at 37 degrees Celsius, the last tube with color change medium as the test bacteria CCU, which is the maximum metabolic activity of mycoplasma, such as the sixth tube appears color change, his relative concentration is 10 6th CCU / ml.
In general, turbidimetry and colony counting method can meet the count of most bacteria, but for mycoplasma such a more specific microorganism, CCU method is more appropriate.
1.1. Constant broth dilution method
1.1.1. Antimicrobial Drug Stock Solutions Antibacterial stock solutions should be prepared at a concentration of no less than 1000 μg / ml (eg 1280 μg / ml) or 10 times the highest measured concentration. Antibacterial drugs with low solubility may be slightly lower than the above concentration. Antimicrobials are purchased directly from manufacturers or related agencies. The required amount of antibacterial solution or powder dosage can be calculated formula. For example, prepare 100 ml of antibiotic stock solution at a concentration of 1280 μg / ml. The antibiotic used is a powder and the potency of the drug is 750 μg / mg. The exact amount of antibiotic powder weighed with the analytical balance was 182.6 mg. Calculate the required amount of diluent according to the formula: (182.6 mg x 750 μg / ml) /1280μg/ml=107.0ml, then dissolve 182.6 mg of antibiotic powder in 107.0 ml of diluent. Table 5 shows the solvents and diluents used to prepare antimicrobial stock solutions. Preparation of a good antibacterial drug storage solution should be stored in the environment below -60 ℃, shelf life of not more than 6 months.
1.1.2. Range of Antimicrobials for Susceptibility Testing According to the NCCLS Antimicrobial Sensitivity Test Operating Standard, the drug concentration range should include values ??for resistance, mediation and sensitive cutoff points, with special exceptions.