禽流感病毒H7亚型核酸PCR检测试剂盒

广州健仑生物科技有限公司

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禽流感病毒H7亚型核酸PCR检测试剂盒

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

物理性污染的预防
● 实验室的合理设计及建立规范的操作规程；
● 培养箱应放在恒温的环境中；
● 培养液及试剂应放在固定位置，而且要注意避光，试剂周围不能放置同位素；
● 从冰箱中取出的培养液应在室温条件中放置一段时间后再进行使用，以避免过冷的温度对细胞的影响。
B.化学性污染的预防
● 使用的所有物质都应是高纯度的，同时正确配制和储存培养液及试剂。
● 在配制液体和清洗容器时必须使用不含杂质的超纯水。
● 选用同一批次的血清。
● 在选用器皿时，根据被培养细胞的生长特性、培养方式和所用培养液的性质来选用适合的器皿来达到实验的准确性和可靠性。
C.生物性污染的预防
● 建立规范的无菌操作程序及各种规章制度，并严格执行。
● 控制环境污染，同时注意实验人员的防护及无菌服的洁净和无菌。
● 保证细胞培养基和器材无菌。
● 在细胞培养基中加入适量的抗生素。
预防是防止细胞培养过程中发生污染的办法。只有提前做好预防工作，才能将发生
污染的可能性降到zui小程度。

Cell culture pollution refers to the invasion of elements which are harmful to the survival of cells and the foreign bodies that cause cell mutation in the culture environment. In vitro cell culture pollution can be divided into three categories: physical, chemical and biological pollution.

Physical pollution:
Physical pollution affects the metabolism of cells by affecting the biochemical components of the cell culture system.
The sources of physical pollution: physical factors in the culture environment, such as temperature, radiation, vibration, radiation (ultraviolet or fluorescence), can affect cells.
The phenomenon of physical pollution: cells, culture fluids or other culture reagents exposed to radiation, radiation or supercooled superheated temperatures can cause changes in cellular metabolism, such as cell synchronization, cell growth inhibition, and even cell death.
Chemical pollution
Chemical pollution is a chemical substance that is toxic to cells or stimulates cells.
Sources of chemical pollution:
Containers of non purified substances, reagents, water, serum, growth cofactors and storage reagents can all be the source of chemical pollution. The essential nutrients (such as amino acids) in cell culture, if the concentration exceeds the reasonable range, can also be toxic to the cells. In the same way, the requirements of different cell lines to the serum and buffer solution are different under the best culture conditions, and should be strictly controlled in the culture.
Chemical pollution: chemical pollution can also inhibit cell growth and even cell death.
Biological pollution
Biological pollution includes contamination of bacteria, fungi, and yeast, which are easier to detect, and the contamination of viruses, mycoplasma and other cells that are more difficult to detect.
Sources of biological pollution:
Inadvertently or mishandled
Air dust
Disinfection is not strict
Improper operation
Reagents, especially serum pollution
The phenomenon of biological pollution:
Bacteria: more G+ bacteria.
Fungi: more fungi.
Mycoplasma: it is not easy to detect.
Virus: the virus has a species specificity, so the probability of virus contamination is relatively small.