犬弓形虫定量检测卡TOXO

广州健仑生物科技有限公司

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动物类的主要检测项目包括：猪、狗、猫、牛、鸡、鸭和鹅的传染病。

畜牧类的主要检测项目包括：多种添加剂、瘦肉精添加剂等。

食品类的主要检测项目包括：添加剂残留、农药残留、药物残留等。

化妆品的主要检测项目包括：重金属、有害物质等。

水产品的主要检测项目包括：呋喃类药物残留、抗生素残留等。

违禁品的主要检测项目包括：MOP/MET/KET/THC/MDMA/COC/BZO/AMP/K2/BAR/TCA/BUP/MTD/PCP/OXY/EDDP

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【犬弓形虫定量检测卡TOXO】

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【公司名称】 广州健仑生物科技有限公司

【市 场 部】    杨永汉

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【腾讯Q Q】 2042552662

【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

1.1.5.稀释抗菌药物的制备及菌液接种 取无菌试管（13×100mm）13支，排成一排，除第1管加入1.6mlMH肉汤外，其余每管加入MH肉汤1ml，在第1管加入抗菌药物原液（如1280μg/ml） 0.4ml混匀，然后吸取1ml至第2管，混匀后再吸取1ml至第3管，如此连续倍比稀释至第11管，并从第11管中吸取1ml弃去，第12管为不含药物的生长对照。此时各管药物浓度依次为256、128、64、32、16、8、4、2、1、0.5、0.25μg/ml。然后在每管内加入上述制备好的接种物各1ml，使每管zui终菌液浓度约为5×105CFU/ml。第1管至第11管药物浓度分别为128、64、32、16、8、4、2、1、05、0.25、0.125μg/ml。
1.1.6.孵育 将接种好的稀释管塞好塞子，置35℃普通空气孵箱中孵育16~20h；嗜血杆菌和链球菌在普通空气孵箱中孵育20~24h；对可能的耐甲氧西林葡萄球菌和耐万古霉素肠球菌应持续孵育满24h。
1.1.7.结果判断与解释 在读取和报告所测试菌株的MIC前，应检查生长对照管的细菌生长情况是否良好，同时还应检查接种物的传代培养情况以确定其是否污染，质控菌株的MIC值是否处于质控范围。以肉眼观察，药物zui低浓度管无细菌生长者，即为受试菌的MIC。甲氧苄胺嘧啶或磺胺药物的肉汤稀释法终点判断，与阳性生长对照管比较抑制80%细菌生长管药物浓度为受试菌MIC。
根据NCCLS推荐的分界点值标准，判断耐药（resistant, R）、敏感(susceptible, S)或中介（intermediate, I）。S表示被测菌株所引起的感染可以用该抗菌药物的常用剂量治疗有效，禁忌症除外。R指该菌不能被抗菌药物的常用剂量在组织液内或血液中所达到的浓度所抑制，或属于具有特定耐药机理（如β-内酰胺酶），所以临床治疗效果不佳。I是指MIC接近药物的血液或组织液浓度，疗效低于敏感菌。还表示被测菌株可以通过提高剂量（如β-内酰胺类药物）被抑制，或在药物生理性浓集的部位（如尿液）被抑制。另外，中介还作为“缓冲域”，以防止由微小的技术因素失控，所导致较大的错误解释。
1.2.微量肉汤稀释法 
1.2.1.抗菌药物和培养基制备 同常量肉汤稀释法。
1.2.2.MIC板制备 无菌操作，将倍比稀释后不同浓度的抗菌药物溶液分别加到灭菌的96孔聚苯乙烯板中，第1至第11孔加药液，每孔10μl，第12孔不加药作为生长对照，冰冻干燥后密封，-20℃以下保存备用。

1.1.5. Preparation of diluted antibacterial drugs and bacterial inoculum take sterile tube (13 × 100mm) 13, arranged in a row, in addition to the first tube to join 1.6mlM broth, the rest of each tube was added MH broth 1ml, In the first tube by adding antibacterial stock solution (such as 1280μg / ml) 0.4ml mix, then draw 1ml to the second tube, mix and then draw 1ml to the third tube, so continuous serial dilution to 11 tubes, and from Pipette 1ml discard the first 11, the first 12 tubes for the drug-free growth control. At this point the drug concentration in each tube were 256,128,64,32,16,8,4,2,1,0.5,0.25μg / ml. Then add 1ml each of the prepared inoculum in each tube so that the final bacterial concentration of each tube is about 5 × 10 5 CFU / ml. The first to the eleventh tube drug concentrations were 128,64,32,16,8,4,2,1,05,0.25,0.125μg / ml.
1.1.6. Incubation Inoculation of a good dilution pipe plug plug, set 35 ℃ normal air incubator incubated 16 ~ 20h; Haemophilus and Streptococcus incubated in an ordinary air incubator 20 ~ 24h; the possible resistance to a Staphylococcus aureus and vancomycin-resistant enterococci should be incubated for 24 hours.
1.1.7. Judgment and interpretation of results Before reading and reporting the MIC of the tested strains, the growth of the bacteria in the growth control should be checked for good bacterial growth, and the inoculum culture should be checked for contamination, control The MIC value of the strain is in the control range. Observed with the naked eye, the minimum concentration of drug-free bacterial growth, that is, the MIC of the test bacteria. Trimethoprim or sulfa drug broth dilution method to determine the endpoint, compared with the positive growth control tube 80% inhibition of bacterial growth tube drug concentration MIC for the test bacteria.
According to the NCCLS recommended cut-off value criteria, it is judged resistant, susceptible, intermediate or I. S indicates that the infection caused by the tested strain can be treated with the usual dose of the antimicrobial agent except contraindications. R means that the bacteria can not be the usual dose of antimicrobial agents in the tissue fluid or blood concentrations reached by inhibition, or belong to a specific resistance mechanism (such as β-lactamase), so the clinical curative effect is not good. I refers to the MIC close to the drug blood or tissue fluid concentration, less effective than sensitive bacteria. It also indicates that the tested strain can be inhibited by increasing doses (such as beta-lactam) or at sites where the drug is physiologically concentrated (such as urine). In addition, the intermediary also acts as a "buffer zone" to prevent large technical errors from being overseen by minor technical considerations.
1.2. Micro broth dilution method
1.2.1. Antibacterial drugs and medium preparation with the same constant broth dilution method.
1.2.2.MIC plate preparation of aseptic operation, the dilution ratio of different concentrations of antibacterial drug solution were added to sterilized 96-well polystyrene plates, the first 11 holes plus liquid, each hole 10μl, The first 12 wells without drug as a growth control, freeze-dried and sealed, -20 ℃ the following reserve.