酚酞检测试剂盒

广州健仑生物科技有限公司

广州健仑长期供应：动物、畜牧、食品、药品、化妆品、水产品、违禁品的快速检测试剂盒。

动物类的主要检测项目包括：猪、狗、猫、牛、鸡、鸭和鹅的传染病。

畜牧类的主要检测项目包括：多种添加剂、瘦肉精添加剂等。

食品类的主要检测项目包括：添加剂残留、农药残留、药物残留等。

化妆品的主要检测项目包括：重金属、有害物质等。

水产品的主要检测项目包括：呋喃类药物残留、抗生素残留等。

违禁品的主要检测项目包括：MOP/MET/KET/THC/MDMA/COC/BZO/AMP/K2/BAR/TCA/BUP/MTD/PCP/OXY/EDDP

我司还提供其它进口或国产试剂盒：包括传染病系列、免疫组化系列、诊断血清等产品。

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酚酞检测试剂盒

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【公司名称】 广州健仑生物科技有限公司

【市 场 部】    杨永汉

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【腾讯Q Q】 2042552662

【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

Reducing agent removal:
Reducing agents are generally slowly oxidized and removed along with the removal of the denaturant so that disulfide bonds form slowly. However, since the formation of disulfide bonds is not necessary for proper protein folding, it is conceivable that after removal of the denaturant, the correct disulfide bonds are formed between the thiol groups which have been brought close to each other in the correct structure after removal of the reducing agent.
Commonly used methods of oxidation are:
Air oxidation: the air in the alkaline conditions, or by adding divalent copper ions, can achieve better results, the disadvantage is not easy to control the redox potential.
Redox: Redox: GSSG / GSH is often used to control the more accurate redox potential by adjusting the ratio between the two. Alternatively, GSSG can be added directly to the solution with reducing agents such as BME and DTT. For example, 5 mM GSSG / 2 mM DTT = GSH / GSSG (1.33 / 1).
Reproducibility efficiency:
Renaturation is a very complex process that, in addition to being related to the process control of protein renaturation, is largely related to the nature of the protein itself, and some of the proteins are very renaturable, such as bovine pancreatic RNase with 12 pairs of disulfide bonds , The refolding efficiency can reach more than 95% under more relaxed conditions. However, some proteins have so far not been found to be capable of refolding such as IL-11, and the refolding efficiency of many proteins is only 0.1%. In general, protein renaturation efficiency of about 20%. Factors that affect the efficiency of renaturation are renaturation concentration of protein, initial concentration and removal rate of denaturant, temperature, pH, redox potential, ionic strength, presence or absence of cosolvent and other additives.