褪黑素快速检测试剂盒

广州健仑生物科技有限公司

广州健仑长期供应：动物、畜牧、食品、药品、化妆品、水产品、违禁品的快速检测试剂盒。

动物类的主要检测项目包括：猪、狗、猫、牛、鸡、鸭和鹅的传染病。

畜牧类的主要检测项目包括：多种添加剂、瘦肉精添加剂等。

食品类的主要检测项目包括：添加剂残留、农药残留、药物残留等。

化妆品的主要检测项目包括：重金属、有害物质等。

水产品的主要检测项目包括：呋喃类药物残留、抗生素残留等。

违禁品的主要检测项目包括：MOP/MET/KET/THC/MDMA/COC/BZO/AMP/K2/BAR/TCA/BUP/MTD/PCP/OXY/EDDP

我司还提供其它进口或国产试剂盒：包括传染病系列、免疫组化系列、诊断血清等产品。

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褪黑素快速检测试剂盒

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【公司名称】 广州健仑生物科技有限公司

【市 场 部】    杨永汉

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【腾讯Q Q】 2042552662

【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

6．胆汁七叶苷水解试验
（1）原理：在10%~40％胆汁存在下，测定细菌水解七叶苷的能力。七叶苷被细菌分解生成的七叶素，七叶素与培养基中的枸橼酸铁的二价铁离子发生反应形成黑色化合物。
(2)方法：将被检菌接种于胆汁七叶苷培养基中，35℃孵育18~24h后，观察结果。
(3)结果：培养基*变黑为阳性，不变黑为阴性。
(4)应用：主要用于鉴别D群链球菌与其它链球菌的区别，以及肠杆菌科的某些种、某些厌氧菌（如脆弱拟杆菌等）的初步鉴别。D群链球菌本试验为阳性。
7．淀粉水解试验
(1)原理：产生淀粉酶的细菌能将淀粉水解为糖类，在培养基上滴加碘液时，可在菌落周围出现透明区。
(2)方法：将被检菌划线接种于淀粉琼脂平板或试管中，35℃孵育18~24h，加入革兰碘液数滴，立即观察结果。
(3)结果：阳性反应，菌落周围有无色透明区，其它地方蓝色；阴性反应，培养基全部为蓝色。
(4)应用：用于白喉棒状杆菌生物型的分型，重型淀粉水解试验阳性，轻、中型阴性；芽胞杆菌属菌种和厌氧菌某些种的鉴定。
8．甘油复红试验
(1)原理：甘油可被细菌分解生成丙酮酸，丙酮酸脱去羧基为乙醛，乙醛与无色的复红生成醌式化合物，呈深紫红色。
(2)方法：取被检菌接种于甘油复红肉汤培养基中，于35℃孵育，观察2~8d。应同时做阴性对照。
(3)结果：紫红色为阳性，与对照管颜色相同为阴性。
(4)应用：主要用于沙门菌属内各菌种间的鉴别。伤寒沙门菌、甲（丙）型副伤寒沙门菌、猪霍乱沙门菌、孔道夫沙门菌和仙台沙门菌本试验为阴性，乙型副伤寒沙门菌结果不定，其它不常见沙门菌多数为阳性。

4. β-galactosidase test (ONPG test)
(1) Principle: lactose permease and β-galactosidase can rapidly decompose in the process of lactose fermentation. Some bacteria only galactosidase, which can only ferment lactose slowly, all the rapid fermentation of lactose and slow fermentation of bacteria can be rapid hydrolysis of o-nitrophenyl-β-D-galactopyranoside (O-nitrophenyl-β-D-galactopyranoside , ONPG) to generate yellow o-nitrophenol. For citric acid bacteria, Arizona and Salmonella genus identification.
(2) Method: Inoculated bacteria to be tested in ONPG broth, incubated at 35 ℃ water bath or incubator for 18 ~ 24h, the results were observed.
(3) Results: showing bright yellow is positive, colorless is negative.
(4) Application: It can be used for the rapid identification of lactic acid bacteria which are slowly fermenting. The bacteria in this method can be positive for bacteria rapidly and slowly decomposing lactose. Escherichia, Citrobacter, Klebsiella, Hafnia, Serratia and Enterobacter are positive tests, and Salmonella, Proteus and Providencia Staphylococcus is negative.
5. VP test
(1) Principle: The ability of bacteria to produce methyl acemethanol was determined. Some bacteria, such as Enterobacter aerogenes, decompose glucose to pyruvate, and pyruvate decarboxylates to form acetylmethylmethanol. Acetylmethylmethanol is oxidized to diacetyl under basic conditions, which in turn combines with guanidine-containing substances such as arginine in the medium to form red compounds. V-P test is positive.
(2) Method: Inoculate the bacteria to be inoculated in glucose peptone water and incubate at 35 ℃ for 24-48h. Add 50ml of α-naphthol (95% ethanol solution) 50ml, shake the tube gently and add 0.2ml 400g / L KOH, gently shake the tube 30s to 1min, and then stand the observation results.
(3) the results: red were positive, yellow or copper-like negative.
(4) Application: Mainly used for the identification of Escherichia coli and Enterobacter aerogenes. This test is often used in conjunction with the MR test, under normal circumstances, the former is a positive bacteria, the latter is often negative, and vice versa. However, the Enterobacteriaceae bacteria do not necessarily have such a law, such as honeycomb Haemophilus and Proteus VP test and MR test are often positive.