MAF/IGH融合基因t(14;16)探针

 广州健仑生物科技?有限公司 

本司长期供应尼古丁（可替宁）检测试剂盒，其主要品牌包括美国NovaBios、广州健仑、广州创仑等进口产品，国产产品，试剂盒的实验方法是胶体金方法。

我司还有很多荧光原位杂交系列检测试剂盒以及各种FISH基因探针和染色体探针等，。

MAF/IGH融合基因t(14;16)探针

   本试剂盒主要用于AML1/ETO融合基因t(8;21)的检测，里面包括即用型杂交液和DAPI复染剂。
本试剂盒仅供科研使用。

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以下是我司出售的部分FISH产品：

MAF/IGH融合基因t(14;16)探针

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【公司名称】 广州健仑生物科技有限公司
【】    杨永汉 

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【腾讯 】
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-3室

【企业文化宣传】MAF/IGH融合基因t(14;16)探针

生殖细胞负责遗传信息的时代传递，那么基因组的完整性对于生殖细胞至关重要。而在真核生物精细胞中，有许多外来侵入的转座子、逆转座子等移动型遗传元件。这些自私的遗传元件在染色体不同位点间跳跃，造成基因突变和基因组损伤。在生殖细胞中，转座子的跳跃可能会导致不育。PiRNA/Piwi能够高效的阻止转座子等元件对基因组的损伤。研究发现，piRNA起源于反转座子、重复序列等区域，与Piwi蛋白形成piRNA/Piwi机器，沉默转座子、反转座子等。此外，piRNA还可以发挥类似于siRNA的功能，参与调控生殖细胞中编码基因的表达。

方法：

研究人员发现无精子症患者体内Piwi（Hiwi）生殖突变会阻止其泛素化和降解。为了了解其中的作用机制，研究人员构建了Piwi（Miwi）突变敲入小鼠模型，证明了这种遗传缺陷直接导致了男性不育症。具体来说，研究人员发现MIWI能以一种对立于Piwi作用RNA（piRNA）的方式，与组蛋白泛素连接酶RNF8结合，并在晚期精子细胞的细胞质中稳定螯合RNF8，从而导致精子异常，引起组蛋白滞留，形态异常和活性严重受损，而这可以通过RNF8-N阻断精子细胞中RNF8-MIWI的相互作用，逆转功能。

研究人员筛查了413例临床无精、弱精症患者Piwi基因上控制Piwi蛋白泛素化修饰降解的关键元件D-box，发现有3例病人在此元件中存在杂合性基因突变，且发现此类突变可来源于基因自发突变，也可从母亲遗传获得。为鉴定此类突变是否是造成这些患者发生无精/少弱精的原因，研究人员将其中的一组突变条件型敲入小鼠Piwi基因，在小鼠模型中研究此类突变对精子发生的作用。他们发现， Piwi D-box杂合突变小鼠均出现雄性不育，精子表型也与患者*。深入研究发现，Miwi D-box杂合突变小鼠精子发生阻滞在延长型精子细胞发育阶段，尽管能产生少量精子，但精子形态异常、细胞核结构疏松、无活力。

机制研究揭示， PIWI蛋白具有将RNF8“扣留”于细胞核外的功能。正常小鼠体内PIWI蛋白会在精子发育后期被自然降解，RNF8进入细胞核内开启“组蛋白-鱼精蛋白转换”，帮助精子发育完成。而Piwi蛋白突变导致其在后期不能被正常代谢，大量RNF8因此被“扣留”在细胞核外，鱼精蛋白与组蛋白交换受阻，zui终造成精子发育受阻。将RNF8-N端导入突变小鼠的精子细胞后，可有效阻断Piwi基因蛋白产物对RNF8的“扣留”，恢复精子的正常形态及游动能力，提示该策略对临床治疗此类无精、弱精症具有重要理论参考价值。

According to foreign media reports, an international research team led by Dirk Schulze-Makuch, Washington State University, found that the most Spartan, seemingly lifeless place on the earth's surface is not so lifeless. Once considered or even lack of microbes, the Atacama Desert showed special bacteria reproduction after rainfall. This indicates that there may be similar "dormancy" groups on red planet.

Atacama Desert is thought to be the earth's closest natural environment to Mars. The living environment of the Chilean desert is so bad that even bacteria can not survive under normal conditions. The rainfall here is measured in millimeters per ten years. Although the 10 million - year history of the desert Mediterranean climate is surprising, there is not enough water to sustain life. Worse, cold temperatures mean less energy that can be used for growth and reproduction.

According to Schulze-Makuch, when the Washington State University's research began in 2015, the unanimate reputation seemed to be intact. Although they found some microbes and DNA traces in Atacama Desert, these residues were proved to come from the outside world.

When the desert first came to rain for decades, the situation changed. It activated the long time dormant biological groups buried under the surface. These groups made full use of a short chance to reproduce. When the research team returned in 2016 and 2017, they found that these populations were gradually dormant when the soil dried up. However, they obtained uncontaminated samples by using sterilized spoon and special instruments, and identified several kinds of local microbial species by genome analysis.

"In the past, researchers have found dead organisms and DNA residues near the surface, but this is the first discovery of persistent life forms in the soil of Atacama Desert," Schulze-Makuch said. "We believe that these microbial communities can be dormant for hundreds of years or even thousands of years on very similar conditions found on planets like Mars, and then return to life when it rains."

Schulze-Makuch believes that the impact on Mars research is quite large.  Though the environment of Mars is so desolate now, billions of years ago, this place is a very different place. There are shallow seas, rivers and lakes, and simple living things have evolved. As Mars becomes colder and drier, it is possible to adapt to some forms of life by long dormancy.

"We know that the water in the Martian soil is frozen, and recent studies strongly show that the night rain and other increased humidity events near the surface," Schulze-Makuch said. "If life has evolved on Mars, our research shows that it may be alive under the dry surface of today."

The Washington State University's research team is scheduled to return to Atacama Desert in March 15th for another two weeks of investigation, which may involve the discovery of more microorganisms. "There are only a few places left on the planet to find new forms of life that can survive on that environment on Mars," Schulze-Makuch said. Our goal is to understand how they do this so that we know what to look for on the surface of Mars. "