丹麦SSI沙门氏菌诊断血清

注意：丹麦SSI诊断血清2018年已涨价

沙门氏菌Vi血清（SSI沙门表面抗原血清）

Vi抗原介绍

表面抗原（即Vi抗原）是一种特殊的菌体抗原，属于K抗原群。

因与毒力有关而命名为Vi抗原。

由聚-n-乙酰-d-半乳糖胺糖醛酸组成。

不稳定，经60°加热，石碳酸处理或人工传代培养易破坏或丢失。

Vi抗原存在于细菌表面，可阻止O抗原与其相应抗体的反应。

Vi抗原的抗原性弱，当体内菌存在时可产生一定量抗体；当细菌被清除后，抗体也随之消失。

Vi抗原血清目录

沙门氏菌Vi血清（SSI沙门表面抗原血清）

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沙门氏菌的抗原分为菌体抗原(O)、鞭毛抗原（H）、荚膜抗原（K、Vi）、纤毛抗原。O抗原O抗原存在于菌体细胞壁zui外层，化学成分为类脂-多糖-多肽复合物，由多糖决定其特异性。O抗原耐热、醇和酸，100～121℃加热2.5h、用乙醇或盐酸处理而不失去抗原性，因此菌体抗原已经被*为沙门氏菌血清型分型的基础。一种菌体有一种或多种不同的O抗原。沙门氏菌的O抗原共有65种，以阿拉伯数字1、2、3……表示。主要抗原：O抗原为某一菌群*而其他菌群不具有。如：2、3、4、7、8、9、10等。次要抗原：O抗原为几个菌群共有。如：1、5、6、12等。 H抗原存在于鞭毛之中，为蛋白质，由肽链中氨基酸的排列顺序及空间构型决定其特异性。不耐热，经加热和用乙醇及碱处理易变性。H抗原常有两相的变异。*相为特异相，用小写英文字母表示，如：a、b、I、e、h等；第二相为非特异相，用阿拉伯数字表示，如：1、2、5等，但也有少数菌含有*相中的抗原e、n、x等成分。 为O抗原表面的荚膜抗原。具有该抗原的细菌，由于可阻止O抗原与抗体结合，因此不被相应的O血清凝集。60℃加热处理或石炭酸处理，虽然该抗原不被灭活，而已从菌体表面脱落，游离于液体中，从而暴露出O抗原，可被O血清凝集。要进行O凝集反应，必须先洗掉Vi抗原。 纤毛抗原是一种比鞭毛更细小的纤维样结构物，含有蛋白性的抗原物质。纤毛能凝集豚鼠的红细胞。所有沙门氏菌的纤毛抗原性一样。纤毛的存在不影响活菌的凝集性，但含有极丰富的纤毛抗原的菌株，有时也组织O的凝集性。

Salmonella antigen is divided into O, flagellum antigen (H), capsular antigen (K, Vi) and cilia antigen. The O antigen of O antigen is found in the outer layer of cell wall. Its chemical composition is lipid polysaccharide polypeptide complex, and its specificity is determined by polysaccharides. O antigen is heat-resistant, alcohol and acid. It is heated for 2.5h at 100~121 C and is treated with ethanol or hydrochloric acid without losing its antigenicity. Therefore, the bacterial antigen has been recognized as the basis of serotype classification of Salmonella. A fungus has one or more different O antigens. There are 65 kinds of O antigens of Salmonella, the number is 1, 2, 3 in Arabia. Express。 The main antigen is O antigen, which is specific for a certain flora but not for other bacteria. Such as: 2, 3, 4, 7, 8, 9, 10, etc. Secondary antigen: O antigen is a group of bacteria. Such as: 1, 5, 6, 12 and so on. H antigen exists in flagellum, which is protein. Its specificity is determined by the sequence and spatial configuration of amino acids in peptide chains. It is heat resistant, heated and treated with ethanol and alkali. H antigen often has two phases of variation. The first phase is a specific phase, which is expressed in lower case English letters, such as a, B, I, e, h, etc.; the second phase is a non specific phase, which is expressed in Arabia numbers, such as 1, 2, 5, etc., but a few bacteria contain the antigens of E, N and X in the first phase. A capsule antigen on the surface of O antigen. The bacteria with this antigen can not bind to the corresponding O serum because it can prevent the binding of O antigen to antibody. Heat treatment at 60 C or carbonic acid treatment, although the antigen is not inactivated, it is removed from the surface of the fungus and free in the liquid to expose the O antigen, which can be agglutinated by the O serum. In order to carry out O agglutination, we must first wash away the Vi antigen. Cilia antigen is a fibrous structure smaller than flagellum, which contains protein antigenic substances. Cilia can agglutinate the red blood cells of the guinea pig. The cilium of all Salmonella is antigenicity. The presence of cilia does not affect the agglutination of live bacteria, but the bacteria that contain very rich cilia antigen sometimes also organizes the agglutination of O.