猪乙脑病毒抗体检测试剂盒（酶联免疫）

广州健仑生物科技有限公司

猪乙脑病毒抗体检测试剂盒（酶联免疫）

【预期用途】 猪乙型脑炎又名日本乙型脑炎是由猪乙脑病毒（Porcine Japanese Encephalitis Virus，JEV）引起的一种蚊媒性、急性人畜共患传染病，病猪主要表现为妊娠母猪流产、产死胎，公猪睾丸肿。本试剂用于检测猪血清中猪乙脑病毒抗体，可用于猪乙脑病毒疫苗免疫效果评价。

【原 理】

 系由预包被猪乙型脑炎病毒重组E2蛋白的酶标板、酶标记物及其他配套试剂组成，应用酶联免疫法（ELISA）原理检测猪血清、血浆样本中猪乙型脑炎病毒抗体。实验时在酶标板中加入对照血清和待检样本，经温育后若样品中含有猪乙型脑炎病毒抗体，则将与酶标板上抗原结合，经洗涤除去未结合的其他成分后；再加入酶标记物，与酶标板上抗原抗体复合物发生特异性结合；再经洗涤除去未结合的酶标记物，在孔中加TMB底物液，与酶反应形成蓝色产物，显色深浅与样品中的特异性抗体含量成正相关；加入终止液终止反应后，产物变为黄色；用酶标仪在450nm波长测定各反应孔中的吸光值，即可知样品是否含有猪乙型脑炎病毒抗体。

【包装规格】 96T×1/盒、96T×2/盒

【储存及有效期】 于2～8℃避光保存，有效期为12个月。

包被板开封后请于2～8℃避光保存，避免受潮。使用期限为2个月

【公司名称】 广州健仑生物科技有限公司

【】 020-825774011- 杨永汉

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【公司】 http://www.jianlun.com

【营销中心】 广州清华科技园番禺区石楼镇创启路63号二期2幢101-103室

我公司同时还有Aflatoxin B1、Aflatoxin、孔雀石绿、了氯霉素、四环素、磺胺多残留、喹诺酮、呋喃西林、玉米赤霉烯酮，呕吐毒素、莱克多巴胺、（Clenbuterol）试剂盒、检测卡等，各种质控，抗原，  杨永汉 同时还有化妆品、保健品类检测。

创仑生物科技有限公司组织旅游活动。使公司的每个成员都能强烈地感受到自己是企业雄伟城墙中的一块砖，是*的一份子。砖与砖之间紧密结合是建立城墙的基础，这种紧密结合就是凝聚力。团队激励图片中的员工凝聚力是企业发展的源泉和集体创造力源泉。

广州健仑生物科技有限公司是集研制开发、销售、服务于一体的优良企业,公司产品涉及临床快速诊断试剂、食品安全检测剂，narcotics快速检测，动物疾病防疫检测试剂，免疫诊断试剂、临床血液学和体液学检验试剂、微生物检验试剂、分子生物学检验试剂、临床生化试剂、有机试剂等众多领域，同时核心代理Panbio、FOCUS、Qiagen、IBL、CORTEZ、Fuller、Inbios、BinaxNOW、LumuQuick、日本富士、日本生研等多家有名诊断产品集团公司产品，致力于为商检单位、疾病预防控制中心、海关出入境检疫局、卫生防疫单位,缉毒系统，戒毒中心，检验检疫单位、生化企业、科研院所、医疗机构等机构与行业提供*、高品质的产品服务。此外，本公司还开展食品、卫生、环境、药品等多方面的第三方检测服务。欢迎致电    杨永汉

日本乙型脑炎又名流行性乙型脑炎，是由日本乙型脑炎病毒引起的一种急性人兽共患传染病。猪主要特征为高热、流产、死胎和公猪睾丸炎。

乙型脑炎是自然疫源性疫病，许多动物感染后可成为本病的传染源，猪的感染zui为普遍。本病主要通过蚊的叮咬进行传播，病毒能在蚊体内繁殖，并可越冬，经卵传递，成为次年感染动物的来源。由于经蚊虫传播，因而流行与蚊虫的孳生及活动有密切关系，有明显的季节性，80%的病例发生在7、8、9三个月；猪的发病年龄与性成熟有关，大多在6月龄左右发病，其特点是感染率高，发病率低(20%-30%),死亡率低；新疫区发病率高，病情严重，

猪只感染乙脑时，临诊上几乎没有脑炎症状的病例；猪常突然发生，体温升至40-41摄氏度，稽留热，病猪精神萎缩，食欲减少或废绝，粪干呈球状，表面附着灰白色粘液；有的猪后肢呈轻度麻痹，步态不稳，关节肿大，跛行；有的病猪视力障碍；zui后麻痹死亡。妊娠母猪突然发生流产，产出死胎、木乃伊和弱胎，母猪无明显异常表现，同胎也见正产胎儿。公猪除有一般症状外，常发生一侧性睾丸肿大，也有两侧性的，患病睾丸皱襞消失、发亮，有热痛感，约经3-5天后肿胀消退，有的睾丸变小变硬，失去配种繁殖能力。如仅一侧发炎，仍有配种能力。

流产胎儿脑水肿，皮下血样浸润，肌肉似水煮样，腹水增多；木乃伊胎儿从拇指大小到正常大??；肝、脾、肾有坏死灶；全身淋巴结出血；肺瘀血、水肿。子宫粘膜充血、出血和有粘液。胎盘水肿或见出血。公猪睾丸实质充血、出血和小坏死灶；睾丸硬化者，体积缩小，实质结缔组织化。

由于本病隐性感染机会多，血清学反应都会出现阳性，需采取双份血清，检查抗体上升情况，结合临诊症状，才有诊断价值。

无治疗方法，一旦确诊淘汰。做好死胎儿、胎盘及分泌物等的处理；驱灭蚊虫，注意消灭越冬蚊；在流行地区猪场，在蚊虫开始活动前1-2个月，对4月龄以上至两岁的公母猪，应用乙型脑炎弱毒疫苗进行预防注射，第二年加强免疫一次，免疫期可达3年。

Japanese encephalitis, also known as epidemic encephalitis b, is an acute zoonotic infectious disease caused by Japanese encephalitis virus. Pigs are mainly characterized by high fever, abortion, stillbirth and boar orchitis.
Japanese encephalitis is a natural epidemic disease. many animals can become the source of infection after infection, and pig infection is the most common. This disease is mainly transmitted through mosquito bites, and the virus can multiply in mosquitoes, overwinter and be transmitted through eggs, becoming the source of infected animals in the following year. As a result of mosquito transmission, epidemic is closely related to mosquito breeding and activities, with obvious seasonality, 80 % cases occurred in 7, 8 and 9 months. The onset age of pigs is related to sexual maturity, and most of them are around 6 months old. its characteristics are high infection rate, low incidence rate ( 20 % - 30 % ) and low mortality rate. The incidence rate of new epidemic areas is high and the disease is serious.
When pigs are infected with Japanese encephalitis, there are almost no encephalitis cases in the clinic. Pigs often happen suddenly, the body temperature rises to 40 - 41 degrees celsius, and the fever is missed, the diseased pigs' spirit shrinks, their appetite is reduced or eliminated, the dung is dry and spherical, and grayish white mucus adheres to the surface. Some pigs showed mild paralysis, unstable gait, swollen joints and limping. Some sick pigs have visual impairment; Finally paralysis and death. Pregnant sows suddenly miscarried, producing stillbirths, mummies and weak fetuses, and sows showed no obvious abnormal performance, and fetuses were also found in the same fetus. Besides general symptoms, boars often have unilateral testicular swelling, and they also have bilateral features. the scrotal folds of diseased testes disappear, shine and feel hot. after about 3 - 5 days, the swelling subsided, and some testes became smaller and stiffer, thus losing the ability of breeding and reproduction. If only one side is inflamed, there is still mating ability.
Fetal brain edema caused by abortion, subcutaneous blood sample infiltration, muscle boiling like, ascites increased; Mummy fetus from thumb size to normal size; Necrotic lesions were found in liver, spleen and kidney. Systemic lymph node hemorrhage; Pulmonary congestion and edema. Hyperemia, hemorrhage and mucus in uterine mucosa. Placental edema or hemorrhage. Congestion, hemorrhage and small necrosis of testicular parenchyma in boars; Testicular sclerosis is characterized by shrinking volume, adhesion with scrotum and substantial connective tissue.
As there are many opportunities for latent infection of this disease, serological reactions will be positive, so it is necessary to take double serum, check the rise of antibody and combine with clinical symptoms, so as to have diagnostic value.
There is no treatment method, once diagnosed, it is best to be eliminated. Do a good job in the treatment of dead fetus, placenta and secretions, etc. Eliminate mosquitoes, pay attention to eliminate overwintering mosquitoes; In pig farms in epidemic areas, sows aged over 4 months to 2 years old are immunized with attenuated Japanese encephalitis vaccine 1 - 2 months before mosquitoes start their activities, and the immunization period can reach 3 years, which is relatively high.